Objective (1)To explore lithium-based bioglass hydrogels and engender mesoporous bioglass biomaterials with multiple biomedical functions such as bone promotion，angiogenesis promotion and lipogenesis inhibition.(2)3D printing technology was used to make navigation templates，accurately locate and remove the lesions of osteonecrosis of the femoral head(ONFH)，and lithium-based biogalglass hydrogel was accurately injected to observe its effect on repairing ONFH.Methods GelMA and lithium bioglass hydrogel (GM/M-Li) were prepared by using Gelatin (Gelatin) and methacrylic anhydride (MA).The materials were characterized by scanning electron microscope (SEM) and transmission electron microscope(TEM).At the same time，the biocompatibility of the material was evaluated by cell alive/dead staining，cell counting kit-8 (CCK-8) and cell morphology observation.In addition，immunofluorescence (IF) staining，alkaline phosphatase (ALP) staining，alizarin red staining (ARS)，angiogenesis assay，and oil red O staining were used to evaluate the osteogenic differentiation ability，angiogenesis ability，and lipogenesis inhibition ability of the materials.A rabbit femoral head necrosis model was successfully established in vivo.
The necrotic bone was removed by 3D navigation template combined with core decompression and then filled with GM/M-Li hydrogel.Hematoxylin eosin(HE) staining，oil red O staining and immunohistochemical staining were used to verify the osteogenesis，angiogenesis and lipogenesis inhibition.Results The results of SEM showed that the GM/M-Li hydrogel has a three-dimensional porous structure.The results of TEM showed that M-Li material was in nanometer scale.The ion release experiments show that the lithium element in GM/M-Li hydrogel has slow release effect.It was proved that GM/M-Li hydrogel had good biocompatibility by cell alive/dead staining and CCK-8.In vitro experiments on osteogenesis，angiogenesis and inhibition of adipogenesis confirmed that the release of lithium ions in the material was conducive to the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCS) and the tubule formation of human umbilical vein vascular endothelial cells (HUVECs)，and inhibited the adipogenesis of adipogenic precursor cells (3T3-L1).In vivo HE staining and IF proved that GM/M-Li hydrogel effectively promoted bone tissue and blood vessel regeneration.The ability of GM/M-Li hydrogel to inhibit fat was verified by oil red O staining.Conclusion GM/M-Li can be accurately injected into the necrotic area of the femoral head and cleared the lesion with 3D printed navigation template.GM/M-Li hydrogel has good biocompatibility and can promote the reconstruction of bone defect in osteonecrosis of the femoral head by regulating BMSCS differentiation in osteogenic direction，promote the formation of blood vessels and reduce the infiltration of fat cells.
马桥桥，吴泽睿，查国春，郭开今，蒋守海，张传开. 导航模板注射锂基生物玻璃水凝胶修复股骨头坏死的效应研究[J]. 实用骨科杂志, 2024, 30(1): 39-.
Ma Qiaoqiao，Wu Zerui，Zha Guochun，Guo Kaijin，Jiang Shouhai，Zhang Chuankai. Study on the Effect of Injection of Lithium Bioglass Hydrogel with Navigation Template to Repair Femoral Head Necrosis. sygkzz, 2024, 30(1): 39-.
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