Abstract:Objective To study the inhibitory effect of MiR-367 on osteoblast proliferation through Wnt/β-catenin pathway.Methods Bone marrow mesenchymal stem cells (BMSCs) were transfected with mimic or inhibitor of miR-367,and osteogenic differentiation was observed by alizarin red staining.Osteoblasts from neonatal rats were cultured and transfected with mimic or inhibitor of miR367,combined with SKL2001 or XAV939 as an agonist or antagonist of β-catenin.Cell proliferation activity was measured by MTT method,and the expression of β-catenin and osteogenic markers gene RUNX2,ALP,OCN,Col-Ⅰ were detected by fluorescence quantitative PCR and Western blot.Results After transfection of mir367 mimics or inhibitor,OD570 value of alizarin red staining had no significant difference with those transfected with NC mimic or inhibitor(P>0.05).OD490 value of MTS and expression levels of β-catenin,RUNX2,ALP,OCN and Col-Ⅰ in osteoblasts of neonatal rats after transfection of miR-367 mimic were significantly lower than those of NC mimic,OD490 value of MTS and expression levels of β-catenin,RUNX2,ALP,OCN and Col-Ⅰ in osteoblasts of neonatal rats after transfection of mir-367 inhibitor were significantly higher than those of NC inhibitor(P<0.05).The expression levels of β-catenin,RUNX2,ALP,OCN and Col-Ⅰ in osteoblasts of newborn rats treated with miR-367 mimic combined with SKL2001 were significantly higher than those of miR-367mimic.The expression levels of β-catenin,RUNX2,ALP,OCN and Col-Ⅰ in osteoblasts of newborn rats treated with miR-367 inhibitor combined with SKL2001 were significantly lower than those of miR-367 inhibitor.Conclusion MiR-367 can inhibit the proliferation of osteoblasts,but does not affect the differentiation of osteoblasts,which may be achieved by inhibiting Wnt/β-catenin pathway.
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