Abstract:Objective To study the osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs)and the repair effects of critical bone defect of rat skull by porous silk fibroin (SF)scaffold loaded with salvianolic acid B (SB).Methods Firstly,0.2mg,1mg and 5mg SB were added into 5ml 10% silk fibroin solution,porous scaffolds (SF/LSB,SF/MSB and SF/HSB)were prepared by freeze-drying method.The release of SB from scaffolds was determined according to the detection wavelength of SB.CCK-8 cell viability test,alkaline phosphatase activity,alizarin red S(ARS) staining and polymerase chain reaction(PCR) were used to detect the proliferation and osteogenic differentiation of rBMSCs.The safe dose group was obtained.The repair effect of SF porous scaffold delivering SB on bone defect was observed by micro-CT scanning,sequence fluorescence labeling and hard tissue section staining.SF group was used as control.Results SB was stably released from the scaffolds,and the released SB promoted the osteogenic differentiation of rBMSCs in a dose-dependent in vitro,but there was no significant difference between SF/MSB and SF/HSB groups.The in vivo experimental results showed that SF/MSB scaffold could significantly promote new bone regeneration and mineralization compared with SF scaffold.Conclusion Delivering SB with SF scaffold can significantly promote the proliferation,osteogenic differentiation of rBMSCs and enhance the repair effect of bone defects.
陆家琪,李晓林 *. 携载丹酚酸B的丝素蛋白支架修复大鼠颅骨缺损的研究[J]. 实用骨科杂志, 2022, 28(4): 317-322.
Lu Jiaqi,Li Xiaolin *. Silk Fibroin Scaffold with Salvianolic Acid B with for Bone Defect of Rat Skull Repairing. sygkzz, 2022, 28(4): 317-322.
[1]Wang L,Zhu L X,Wang Z,et al.Development of a centrally vascularized tissue engineering bone graft with the unique core-shell composite structure for large femoral bone defect treatment[J].Biomaterials,2018(175):44-60.
[2]Sen MK,Miclau T.Autologous iliac crest bone graft:Should it still be the gold standard for treating nonunions?[J].Injury,2007,38(suppl 1):S75-S80.
[3]Balogh ZJ,Reumann MK,Gruen RL,et al.Advances and future directions for management of trauma patients with musculoskeletal injuries[J].Lancet,2012,380(9847):1109-1119.
[4]Myojin K,Miji Y,Minseong K,et al.Biomimetic cellulose/calcium-deficient-hydroxyapatite composite scaffolds fabricated using an electric field for bone tissue engineering[J].RSC Advances,2018,8(37):20637-20647.
[5]Wu J,Zheng A,Liu Y,et al.Enhanced bone regeneration of the silk fibroin electrospun scaffolds through the modification of the graphene oxide functionalized by BMP-2 peptide[J].Int J Nanomedicine,2019(14):733-751.
[6]Wu J,Zheng K,Huang X,et al.Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects[J].Acta Biomater,2019(91):60-71.
[7]Wang Q,Zhang Y,Li B,et al.Controlled dual delivery of low doses of BMP-2 and VEGF in a silk fibroin-nanohydroxyapatite scaffold for vascularized bone regeneration[J].J Mater Chem B,2017,5(33):6963-6972.
[8]Zhou L,Zuo Z,Chow MS.Danshen:an overview of its chemistry,pharmacology,pharmacokinetics,and clinical use[J].J Clin Pharmacol,2005,45(12):1345-1359.
[9]Orgah JO,He S,Wang Y,et al.Pharmacological potential of the combination of Salvia miltiorrhiza (Danshen)and Carthamus tinctorius (Honghua)for diabetes mellitus and its cardiovascular complications[J].Pharmacol Res,2020(153):104654.
[10]Fan Y,Luo Q,Wei J,et al.Mechanism of salvianolic acid B neuroprotection against ischemia/reperfusion induced cerebral injury[J].Brain Res,2018(1679):125-133.
[11]Xu D,Xu L,Zhou C,et al.Salvianolic acid B promotes osteogenesis of human mesenchymal stem cells through activating ERK signaling pathway[J].Int J Biochem Cell Biol,2014(51):1-9.
[12]Zheng A,Cao L,Liu Y,et al.Biocompatible silk/calcium silicate/sodium alginate composite scaffolds for bone tissue engineering[J].Carbohydr Polym,2018(199):244-255.
[13]Qi X,Liu Y,Ding Z Y,et al.Synergistic effects of dimethyloxallyl glycine and recombinant human bone morphogenetic protein-2 on repair of critical-sized bone defects in rats[J].Sci Rep,2017(7):42820.
[14]Liu W,Li J,Cheng M,et al.Zincmodified sulfonated polyetheretherketone surface with immunomodulatory function for guiding cell fate and bone regeneration[J].Adv Sci (Weinh),2018,5(10):1800749.
[15]Place ES,Evans ND,Stevens MM.Complexity in biomaterials for tissue engineering[J].Nature materials,2009,8(6):457-470.
[16]Gautschi OP,Frey SP,Zellweger R.Bone morphogenetic proteins in clinical applications[J].ANZ J Surg,2007,77(8):626-631.
[17]Lin S,Cui L,Chen G,et al.PLGA/beta-TCP composite scaffold incorporating salvianolic acid B promotes bone fusion by angiogenesis and osteogenesis in a rat spinal fusion model[J].Biomaterials,2019(196):109-121.
[18]Fan Z,Wang J,Liu F,et al.A new composite scaffold of bioactive glass nanoparticles/graphene:Synchronous improvements of cytocompatibility and mechanical property[J].Colloids Surf B Biointerfaces,2016(145):438-446.
[19]Hao D,Danbin W,Maojuan G,et al.Ethanol extracts of danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent[J].Pharmacol Res,2019(146):104306.
[20]Cui L,Li T,Liu YY,et al.Salvianolic acid B prevents bone loss in prednisone-treated rats through stimulation of osteogenesis and bone marrow angiogenesis[J].PLoS One,2012,7(4):e34647.