The Characterization and Histocompatibility of 3D Printed PLLA/β-TCP Composite Artificial Bone
1.Department of Orthopedics,Guizhou Orthopedics Hospital
2.The First People’s Hospital of GuiYang
3.Department of Orthopaedic,Children’s Hospital of Chongqing Medical University
Abstract:Objective To prepare the Poly(L-lactic acid)/β- tricalcium phosphate(PLLA/β-TCP)composite artificial boneutilizing 3D printing technologyand investigate its physical properties and histocompatibility in vivo to verify its feasibility as tissue engineered bone materials.Methods The PLLA/β-TCP composite artificial bones were fabricated by 3D printing technology,their microstructure was observed by Scanning electron microscopy (SEM),their physical strength was detected by dynamic and static fatigue testing machine.Their porosity was measured by Mercury porosimeter and densimetry.The state of crawling C3H10 cells in composite artificial bones were observed by SEM to evaluate the cytocompatibility of artificial bones.The leaching solution of composite artificial bones was used to culture the C3H10 cells and observing the effect of it on cell differentiation.The histocompatibility of PLLA/ β-TCP composite artificial bones implanted in the gluteus muscle mass of New Zealand rabbits were observed after one month,two months and three months.Results SEM showed that PLLA/β-TCP had a porous structure.The C3H10 cells cultured in PLLA/ β-TCP composite artificial bone were fully extended and energetic,which indicating PLLA/β- TCP composited artificial bones had good cellular compatibility.The maximum elasticity modulus of PLLA/ β-TCP composite artificial bones was (21.1±3.4)MPa and the maximum bending strength was (9.5±2.3)MPa,the porosity of PLLA/β-TCP composited artificial bone we needed was (51±3)%.The alkaline phosphatase staining and alizarin red staining were performed after C3H10 cells cultured at the seventh day and twenty-first day,the positive results indicated that there was osteoinductive composition in the artificial bone.The tissue sections showed that the artificial bones were surrounded by a layer of loose and dropsical fibrous tissue when PLLA/β-TCP composited artificial bone were implanted in muscle bags at early stage,in which we could find a small amount of neutrophils and lymphocytes.The artificial bone was closely associated with the surrounding tissues and the boundary was blurred,we just observed a little amount of lymphocytes in fiber bundles in the late stage.The experimental results were in accordance with the standard of local reaction after implantation of national medical device.Conclusion 3D printed PLLA/β-TCP composite and porous artificial bone has excellent mechanical strength,good cell compatibility,osteoinduction and histocompatibility,which is an ideal tissue engineered bone material.
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