Abstract:Objective To explore the relationship between the polymorphism of PTH gene rs1459015 and bone mineral density(BMD) of osteoporotic fracture in Han nationality in Beijing.Methods The subjects included 127 osteoporotic fracture(fracture group) and 145 non-osteoporotic fracture(control group).All subjects living in Beijing are Han Chinese without relationship.PTH gene polymorphisms were identified by SNaPshot.BMDs of the lumbar spine (L2~4) and proximal femur including neck,Ward’s,triangle troch and total hip were measured by dual energy X-ray absorptiometry.Allele frequency and genotype frequency were calculated.The BMD difference of two groups and the relationship of the PTH gene polymorphism with BMD of each location in fracture group were analyzed.Results The A and G allele frequency in two groups were 19.7%,80.3% and 18.6%,81.4%,without statistical significance(P>0.05).AA、AG、GG genotype frequencies of two populations were 3.9%,31.5%,64.6% and 3.4%,30.3%,66.2%,and this difference was not statistically significant(P>0.05).The BMDs of each location in fracture group were lower than those in control group,and the differences were statistically significant(P<0.05),not including the difference of BMD of neck.The BMDs of GG genotype were higher than those of AA and AG genotype in fracture group (P>0.05).Compared with AA and GG genotypes in fracture group,AG genotype had higher BMD of hip.Conclusion The PTH gene polymorphism is not associated with BMD of osteoporotic fracture.
[1]Abukhadir SS,Mohamed N,Mohamed N.Pathogenesis of alcohol-induced osteoporosis and its treatment:a review[J].Curr Drug Targets,2013,14(13):1601-1610.
[2]Hwang JS,Chan DC,Chen JF,et al.Clinical practice guidelines for the prevention and treatment of osteoporosis in Taiwan:summary[J].J Bone Miner Metab,2014,32(1):10-16.
[3]钟妮,张红,伍贤平,等.甲状旁腺激素基因多态性与女性骨量及骨相关生化指标的关系[J].中华医学杂志,2006,86(6):376-379.
[4]Kanaya K,Kato Y,Murata Y,et al.Low parathyroid hormone levels in patients who underwent/would undergo hemodialysis result in bone graft failure after posterolateral fusion[J].Spine (Phila Pa 1976),2014,39(4):327-331.
[5]杨艳,李蓬秋,朱显军,等.重组人甲状旁腺激素(1-34)和依降钙素治疗原发性骨质疏松症的随机对照研究[J].中国骨质疏松杂志,2014,20(2):137-141.
[6]Punda M,Grazio S.Bone densitometry the gold standard for diagnosis of osteoporosis[J].Reumatizam,2014,61(2):70-74.
[7]Alarkawi D,Bliuc D,Nguyen TV,et al.Contribution of lumbar spine BMD to fracture risk in individuals with T-score discordance[J].J Bone Miner Res,2016,31(2):274-280.
[8]Hosoi T,Miyao M,Inoue S,et al.Association study of parathyroid hormone gene polymorphism and bone mineral density in Japanese postmenopausal women[J].Calcif Tissue Int,1999,64(3):205-208.
[9]Kanzawa M,Sugimoto T,Kobayashi T,et al.Parathyroid hormone gene polymorphisms in primary hyperparathyroidism[J].Clin Endocrinol (Oxf),1999,50(5):583-588.
[10]何斌,李东风,吴文,等.甲状旁腺激素基因多态性对广东地区老年人骨密度的影响[J].实用医学杂志,2011,27(12):2257-2260.
[11]李东,董兆文,薛延,等.绝经后健康妇女甲状旁腺激素基因多态性与骨密度的关系[J].遗传,2003,25(2):133-136.
[12]Aldaghri NM,Alattas OS,Krishnaswamy S,et al.Association between promoter region genetic variants of PTH SNPs and serum 25(OH)-vitamin D level.[J].Int J Clin Exp Pathol,2015,8(7):8463-8471.
[13]Cappola AR,Shoback DM.Osteoporosis Therapy in Postmenopausal Women With High Risk of Fracture[J].JAMA,2016,316(7):715-716.