Clinical Study of Autologous Adipose Derived Mesenchymal Stem Cells in the Treatment of Osteoarthritis
1.Department of Orthopedics,the Second Clinical Medical College of Lanzhou University
2.The Orthopedics of The First People’s Hospital of Xianyang City
Abstract:Objective To discusses the safety and effectiveness of the treatment injection for knee osteoarthritis by using a utologous ADSCS composite sodium hyaluronate,platelet rich plasma (PRP).Methods Take the abdominal adipose tissue,get hADSCs separation,plenty of ADSCS obtained with in vitro culture,and by flow cytometry instrument to detect the surface markers and analysis into cartilage cells differentiation ability.30 patients with mild-to-moderate of knee joint osteoarthritis were selected from the orthopedic outpatient service center in the first people’s hospital of Xianyang city.ADSCs composite sodium hyaluronate,platelet rich plasma were injected into patient’s articular cavity.The indicators such as patients with adverse reactions,knee activity score,pain visual analogue scale (VAS) score as well as the surface of knee join under MRI were evaluated before and after the intervention.Results Abdominal adipose tissue separated hADSCs were positive expression of CD105 and CD29,CD45,CD34 negative expression,in vitro with good osteogenesis,into fat,cartilage capacity;There was no adverse reaction in the patients who suffered clinical intervention.And patient’s knee pain relief,activity range was improved compared with before treatment.MRI imaging showed that the patchy shadow in the area of the joint surface and low signal area were significantly reduced.There was no obvious joint effusion,and the thickness of articular surface was increased.Conclusion The articular cavity injection therapy with ADSCs compound sodium hyaluronate,platelet rich plasma (PRP) for patients who suffered with osteoarthritis can effectively improved the knee joint motion,to reduce pain and improve the quality of life.The thickness of the joint surface was increased and the joint degeneration was delayed by MRI imaging.
[1]钱春美,沈艳婷,袁秀荣,等.骨关节炎研究进展[J].吉林中医药,2016,36(5):538-540.
[2]张柏青,张仲文,叶启彬.基质诱导自体软骨细胞移植与软骨缺损的修复[J].中国矫形外科杂志,2008,16(23):1796-1798.
[3]美丽,刘少英,刘福艳,等.脂肪组织来源干细胞治疗骨关节炎研究进展[J].国际骨科学杂志,2016,37(2):102-105.
[4]Fischbach C,Seufert JH,Hacker M,et al.Three-dimensional in vitro model of adipogenesis:comparison of culture conditions.[J].Tissue Engineering,2004,10(1-2):215-229.
[5]Fischbach C,Spruss TB,Neubauer M,et al.Generation of mature fat pads in vitro and in vivo utilizing 3-D long-term culture of 3 T3~L1 preadipocytes[J].Experimental Cell Research,2004,300(1):54-64.
[6]Brittberg M,Lindahl A,Nilsson A,et al.Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation[J].N Engl J Med,1994,331(14):889-895.
[7]Mitsuhiko Sato,Kenzo Uchida,Hideaki Nakajima,et al.Direct transplantation of mesenchymal stem cells into the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis.[J].Arthritis Research & Therapy,2012,14(1):R31
[8]Al Faqeh H,Bin Nor Hamdan BM,Chen HC.The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model[J].Experimental Gerontology,2012,47(6):458-464.
[9]Huang JI,Zuk PA,Jones NF,et al.Chondrogenic potential of multipotential cells from human adipose tissue.[J].Plastic & Reconstructive Surgery,2004,113(2):585-594.
[10]Cowan CM,Shi YY,Aalami OO,et al.Adipose-derived adult stromal cells heal critical-size mouse calvarial defects[J].Nature Biotechnology,2004,22(5):560-567.
[11]Zuk P A,Zhu M,Ashjian P,et al.Human adipose tissue is a source of multipotent stem cells[J].Molecular Biology of the Cell,2003,13(12):4279-4295.