Abstract：Objective To investigate the early healing outcomes of tendons and bones in huge rotator cuff defect of rabbits treated with autologous hamstring tendon. Methods Forty male New Zealand white rabbits were randomly divided into three groups：normal group(n＝8)，model group(n＝8)and experimental group(n＝24).The normal group was rfed in the same manner but was not treated.In the model group，a 1.5 cm×1.0 cm rotator cuff defect was made by cutting from the greater tuberosity of humeral head of both forelegs of the rabbits and the skin was directly closed without suture repair.In the experimental group，the huge rotator cuff defect was further repaired by suturing with autologous hamstring tendon on the basis of the model group.8 white rabbits were sacrificed at 8，16，and 24 weeks after surgery in the experimental group and specimens of rotator cuff in both forelegs were collected，which were processed for histomorphological analysis and biomechanical research tests.Results Histomorphological analysis showed that collagen fibers increased significantly at the tendon-bone interface at 24 weeks after the operation in the experimental group.Healing components like Sharpey fibers，fibrochondrocytes，and fibrocartilage were found at the tendon-bone junction of the cut.Biomechanical analysis showed that the maximum load of tendons in the experimental group continued to increase with time(comparison between time points，P＜0.05)，and strong mechanical strength could be obtained after 24 weeks.Conclusion The application of autologous hamstring tendon to repair huge rotator cuff defects can lead to satisfactory tendon bone healing outcomes in rabbits.
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