Abstract:Objective To investigate the effect of miR-96 on proliferation and invasion of osteosarcoma MG63 cell line by interfering with naked cuticle homolog 2 (NKD2) expression.Methods The clinical data of 57 osteosarcoma specimens collected in our hospital from September 2013 to February 2018 were retrospectively analyzed as the study group,and the adjacent tissues 5cm away from the tumor boundary were collected as the control group,including 39 males and 18 females.The age ranged from 21 to 49,with an average age of (28.16±6.73).Osteosarcoma MG63 cells were divided into miR-96 inhibition group and blank control group.After 48 hours of culture,the expression levels of miR-96 and nkd2 in MG63 cells were detected by RT-qPCR and Western blot.MTT method was used to detect the cell proliferation.Cell apoptosis and cell cycle distribution were detected by flow cytometry.Results The expression level of miR-96 in osteos arcoma tissue was significantly higher than that in normal bone tissue (P<0.05).The expression level of NKD2 protein in osteosarcoma tissue was significantly lower than that in normal bone tissue (P<0.05).The expression level of miR-96 in osteosarcoma cells in the miR-96 inhibition group was significantly lower than that in the blank control group (P<0.05).The expression level of NKD2 in osteosarcoma cells in miR-96 inhibition group was significantly higher than that in the blank control group (P<0.05).The relative absorbance of osteosarcoma MG63 cell lines in the miR-96 inhibition group was significantly lower than that in the blank control group (P<0.05).The apoptosis rate of osteosarcoma MG63 cell line inmiR-96 inhibition group was significantly higher than that in the blank control group (P<0.05).The cell cycle of osteosarcoma MG63 cell line was significantly different between the miR-96 inhibition group and the blank control group (P<0.05).Conclusion miR-96 is highly expressed in osteosarcoma,and NKD2 is down-regulated in osteosarcoma.miR-96 promotes the growth and invasion of osteosarcoma MG63 cell line by inhibiting NKD2 expression,thus inhibits early apoptosis of osteosarcoma MG63 cell line.
张建业,张卫华. miR-96干扰NKD2表达对骨肉瘤MG63细胞株的增殖、凋亡的影响[J]. 实用骨科杂志, 2021, 27(7): 615-631.
Zhang Jianye,Zhang Weihua. Effect of miR-96 on Proliferation and Invasion of Osteosarcoma MG63 Cell Line by Interfering with NKD2 Expression. sygkzz, 2021, 27(7): 615-631.
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