Abstract:Objective To investigate the effect of miRNA-27α regulating peroxisome proliferator-activated receptor (PPARγ)/apolipoprotein A5(ApoA5) pathway on steroid-induced femoral head necrosis in rats induced by methylprednisolone.Methods Thirty female SPF-grade SD rats were divided into 5 groups according to the random number method,with 6 in each group.1 group was a blank control group;4 groups were model groups.An in vitro model of femoral head necrosis was induced by methylprednisolone.Immunohistochemical analysis of bone morphology,qRT-PCR analysis of gene expression changes,and MTT method were used to detect cell proliferation and viability.Results Hormone-induced rat femoral head sections showed sparse,broken,and disordered bone trabeculae,significantly reduced the area of bone trabeculae,and significantly reduced the content of miRNA-27α (P<0.05).The levels of PPARγ and ApoA5 mRNA expression were significant increased (P<0.05).PPARγ and ApoA5 showed a negative correlation with miRNA-27α,with correlation coefficients of 0.725 and 0.683,respectively.In the injection group of methylprednisolone and miRNA-27α mimics,the levels of PPARγ and ApoA5 mRNA significantly decreased (P<0.05);the proliferation of miRNA-27α mimics group was significantly higher than that of miRNA-27α inhibitor group.The chondrocyte survival rate in the model group was significantly reduced,but the miRNA-27α inhibitor group also produced a greater inhibitory effect.Conclusion miRNA-27α can delay the progress of hormonal femoral head necrosis by inhibiting the expression of PPARγ / ApoA5.
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