miR-429在低氧环境中对MC3T3-E1细胞成骨分化的影响

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Abstract Objective To investigate the effect of miR-429 on the osteogenic differentiation of MC3T3-E1 cells in a hypoxic environment.Methods The hypoxia environment was simulated by Cobalt chloride (CoCl-2)in vitro and in vivo，the expression of hypoxia-inducible factor-1α (HIF-1α)was detected by Western blotting to verify the hypoxia condition.The expression of miR-429 in cell tissues and the fracture site was observed by real time-quantitative polymerase chain reaction，(RT-qPCR).The proliferation and apoptosis of MC3T3-E1 cells under hypoxia were detected.miR-429 was overexpressed in MC3T3-E1 cells by lentiviral transfection technology as the experimental group and the control group was transfected with empty plasmid cells，and alkaline phosphatase (ALP)activity detection，alizarin red staining，western blotting and other methods were used to observe the ALP activity，mineral content and protein expression of various osteogenic markers in cells to verify the osteogenic differentiation of MC3T3-E1 cells.The fracture model was injected locally with a lentiviral vector overexpressing miR-429，and the fracture healing was observed by Micro-CT and qRT-PCR.Results The high expression of HIF-1α in MC3T3-E1 cells in a hypoxic environment up-regulated the expression of miR-429.After MC3T3-E1 cells were transfected with miR-429 overexpressing virus，the activity of ALP in the cells，the mineralization of cell matrix and the levels of osteogenic markers (RUNX2 and OC)were higher than those in the control group.After injection of lentiviral reagents overexpressing miR-429 in vivo，the Micro-CT of fracture healing were better and the expression of miR-429 in bone tissue were higher than those in the control group.Conclusion MiR-429 is highly expressed in hypoxic environment and plays a role in inducing osteogenic differentiation of MC3T3-E1 cells.

Key words： hypoxia inducible factor-1α miR-429 osteogenic differentiation

Corresponding Authors: Cao Guanglei

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	Huang Jiang
	Jiao Xufeng
	An Shuai
	Wang Juyong
	Cao Guanglei
	Shen Huiliang

Cite this article:

Huang Jiang,Jiao Xufeng,An Shuai, et al. Effect of miR-429 on Osteogenic Differentiation of MC3T3-E1 Cells in a Hypoxic Environment[J]. sygkzz, 2022, 28(8): 712-716.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2022/V28/I8/712

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