携载丹酚酸B的丝素蛋白支架修复大鼠颅骨缺损的研究

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Abstract Objective To study the osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs)and the repair effects of critical bone defect of rat skull by porous silk fibroin (SF)scaffold loaded with salvianolic acid B (SB).Methods Firstly，0.2mg，1mg and 5mg SB were added into 5ml 10% silk fibroin solution，porous scaffolds (SF/LSB，SF/MSB and SF/HSB)were prepared by freeze-drying method.The release of SB from scaffolds was determined according to the detection wavelength of SB.CCK-8 cell viability test，alkaline phosphatase activity，alizarin red S(ARS) staining and polymerase chain reaction(PCR) were used to detect the proliferation and osteogenic differentiation of rBMSCs.The safe dose group was obtained.The repair effect of SF porous scaffold delivering SB on bone defect was observed by micro-CT scanning，sequence fluorescence labeling and hard tissue section staining.SF group was used as control.Results SB was stably released from the scaffolds，and the released SB promoted the osteogenic differentiation of rBMSCs in a dose-dependent in vitro，but there was no significant difference between SF/MSB and SF/HSB groups.The in vivo experimental results showed that SF/MSB scaffold could significantly promote new bone regeneration and mineralization compared with SF scaffold.Conclusion Delivering SB with SF scaffold can significantly promote the proliferation，osteogenic differentiation of rBMSCs and enhance the repair effect of bone defects.

Key words： salvianolic acid B silk fibroin osteogenic differentiation bone regeneration

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	Lu Jiaqi
	Li Xiaolin *

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Lu Jiaqi,Li Xiaolin *. Silk Fibroin Scaffold with Salvianolic Acid B with for Bone Defect of Rat Skull Repairing[J]. sygkzz, 2022, 28(4): 317-322.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2022/V28/I4/317

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