脂联素对骨质疏松大鼠椎体磷酸钙骨水泥强化后骨质量及生物力学的影响及机制

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Abstract Objective To study the effect and mechanism of adiponectin(APN)on bone quality and biomechanics of vertebral body strengthened with calcium phosphate cement(CPC) in osteoporosis rats.Methods Thirty osteoporosis model rats were prepared by bilateral oophorectomy.They were randomly divided into control group，1 mg/kg APN group and 2 mg/kg APN group.All rats were strengthened with CPC of the fourth lumbar vertebra.7 days later，they were given local injection of normal saline，1 mg/kg APN and 2mg/kg APN respectively for 4 weeks.Bone mineral density，trabecular volume fraction(BV/TV)，average trabecular thickness(Tb.Th)，average trabecular number(Tb.N)，maximum coincidence，stiffness and relative expression levels of alkaline phosphatase(ALP)，osteocalcin(OCN)，osteopontin(OPN)，phosphorylated p38MAPK(pp38 MAPK)，phosphorylated JNK(pJNK)，phosphorylated ERK1/2(pERK1/2)were measured.Results Bone mineral density，BV/TB，Tb.Th，Tb.N，maximum coincidence，stiffness and mRNA relative expression levels of ALP，OCN，OPN and protein relative expression levels of ALP，OCN，OPN，p-p38mpak，p-JNK and p-ERK1/2 were higher than those of the control group.Conclusion APN enhances the bone mass and biomechanical strength of vertebral body strengthened with calcium phosphate cement in osteoporosis rats，and the activation of p38MAPK，JNK，ERK1/2 pathways are related molecular mechanisms.

Key words： osteoporosis vertebral compression fracture calcium phosphate bone cement adiponectin signal pathway

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