肩袖损伤患者肌腱和骨组织中氧化应激状态及Beclin1、mTOR的表达分析

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Abstract Objective Tobserve the oxidative stress response and the differential expression of Beclin1 and mTOR in the bone tissues of the tendon and the footprint area of the rotator cuff when the rotator cuff injury occurs.Methods Twenty patients with rotator cuff injury were selected，including 10 males and 10 females.The average age was(61.52±7.32).All patients underwent shoulder arthroscopic rotator cuff anchor repair.The injured tendon tissue and bone tissue samples at rotator cuff insertion were taken as the injured group，and the normal tendon tissue and bone tissue samples beside the footmark were taken as the control group.The DCFHDA method was used to detect the total reactive oxygen species(ROS) level in the tendon and bone tissue samples，the DHE method was used to detect the superoxide dismutase(SOD) content；the RTPCR technique was used to detect the expression of Beclin1 and mTOR mRNA in the tendon and bone tissue.Western blot(WB) detects Beclin1，p-mTOR/mTOR protein expression.Results Compared with the control group，in the injured group，total reactive oxygen species(ROS)，Beclin1 gene and protein were up-regulated(P＜0.05)，mTOR gene and p-mTOR protein were down-regulated(P＜0.05)，and superoxide dismutase(SOD)was down-regulated in the tendon and up-regulated in the bone tissue(P＜0.05).mTOR protein in the injury group was not significantly up-regulated(P＞0.05).Conclusion Rotator cuff injury stimulates the oxidative stress response in the tendon and in the bone tissue of rotator cuff stop point and foot print area，and can also regulate the expression of autophagy factors Beclin1 and mTOR.It affects the healing of the rotator cuff，indicating oxidative stress and autophagy，and has an important regulatory effect on tendonbone healing.

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