IL-15Rα及RUNX2基因多态性与内蒙古地区蒙古族后纵韧带骨化的相关性研究

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Abstract Objective To study polymorphic loci in RUNX2 and IL-15Rα and explore the relationtion betweenpolymorphic loci in RUNX2 and IL-15Rα and OPLL of Mongolian in Inner Mongolia.Methods From January 2014 to December 2019，Mongolian OPLL patients and Mongolian people with physical examination in the same period were selected as the research objects.Gene polymorphism of two candidate genes RUNX2 and IL-15Rα were detected by sequencing in 110 OPLL Mongolian patients.There were 75 males and 35 females.The average age was(53.0±12.8)years old.Control group included 118 healthy Mongolian people，including 65 males and 53 females.The average age was(58.0±11.2)years old.The result of sequencing of patients were compared with control subjects to screen loci with significant difference.Results The frequencies of rs1321075 genotype and allele were compared between 118 healthy Mongolian and 110 Mongolian OPLL patients by chi square test.The differences of AC and CC genotype were statistically significant(P< 0.05)，The OR value(95% CI)was 0.584(0.343，0.997)，1.978(1.166，3.353)，the OR value(95% CI)of allele C was 0.588(0.386，0.895)，the difference was statistically significant.Compared with the genotype and allele frequency of rs16873379，the differences of CT and TT were statistically significant(P<0.05)，the OR value(95% CI)was 2.756(1.595，4.760)，0.266(0.153，0.461)，respectively.The OR value of allele C(95% CI)was 2.514(1.678，3.768)；the difference of genotype and allele frequency of rs2296139 was statistically significant(P<0.05)，OR value(95% CI)was 0.416(0.218，0.797)，and the difference of genotype and allele frequency of other loci was not statistically significant(P＞0.05).Conclusion Polymorphism of rs1321075 and rs2296139 in RUNX2 and IL-15Rα may be responsible for OPLL in Mongolian population patients in Inner Mongolia.

Key words： interleukin-15 receptor alpha recombinant Runt related transcription factor 2 ossification of posterior longitudinal ligament gene polymorphism mongolian group

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	Li Pengfei
	Zhu Shufen *

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Li Pengfei,Zhu Shufen *. IL-15Rα and RUNX2 Polymorphisms Associated with OPLL in the Mongolian Population[J]. sygkzz, 2021, 27(6): 517-533.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2021/V27/I6/517

［1］Goto S，Kita T.Long-term follow up evaluation of surgery for ossificationof the posterior longitudinal ligament［J］.Spine，1995，20(20)：2247-2256.
［2］Matsunaga S，Sakou T，Taketomi E，et al.The natual course of myelopathyby ossification of the posterior longitudinal ligament in the cervical spine［J］.Clin Orthp Relat Res，1994(305)：168-177.
［3］Muthukumar N，Karuppaswamy U.Tumoral calcium pyrophosphate dehydrate deposition disease of the ligamentum flavum ［J］.Neurosurgery，2003，53(1)：103-109.
［4］Inamasu J，Guiot BH，Sachs DC.Ossification of the posterior longitudinal ligament：an update on its biology，epidemiology，and naturalhistory ［J］.Neurosurgery，2006，58(6)：1027-1039.
［5］Wang PN，Chen SS，Liu HC，et al.Ossification of the posterior longitudinal ligament of the spine.A case-control risk factor study ［J］.Spine，1999，24(2)：142-145.
［6］李中实，张光铂，绳厚福，等.我国北方地区颈肩痛病人中颈椎后纵韧带骨化症发病率调查［J］.中国脊柱脊髓杂志，1999，9(5)：285-286.
［7］顾宇彤，贾连顺.颈椎后纵韧带骨化症发病机制的研究进展［J］.中国脊柱脊髓杂志，2004，14(19)：2549-2552.
［8］Terayama K.Genetic studies on ossification of the posterior longitudinal ligament of the spine［J］.Spine，1989，14(11)：1184-1191.
［9］Tanaka T，Ikari K，Furushima K，et al.Genomewide linkage andlinkage disequilibrium analyses identify COL6A1，on chromosome 21，as the locus for ossification of the posterior longitudinal ligamentof the spine ［J］.Am J Hum Genet，2003，73(4)：812-822.
［10］Horikoshi T，Maeda K，Kawaguchi Y，et al.A largescale genetic association study ofossification of the posterior longitudinal ligament of the spine［J］.Hum Genet，2006，119(6)：611-616.
［11］陈欣，孙宇，张凤山，等.目标基因测序技术检测后纵韧带骨化致病基因突变［J］中国矫形外科杂志，2016，24(3)：270-273.
［12］Komori T.Runx2，an inducer of osteoblast and chondrocyte differentiation［J］.Histochem Cell Biol，2018，149(4)：313-323.
［13］Kishiya M，Sawada T，Kanemaru K，et al.A functional RNAi screen for Runx2-regulated genes associated with ectopic bone formation in human spinal ligaments［J］.J Pharmacol Sci，2008，106(3)：404-414.
［14］Liu Y，Zhao YF，Chen Y，et al.Runx2 polymorphisms association with OPLL and OLF in the han population［J］.Clin Orthop Relat Res，2010(468)：3333-3341.
［15］Crane JD，MacNeil LG，Lally JS，et al.Exercise-stimulated interleukin-15 is controlled by AMPK and regulates skin metabolism and aging［J］.Aging Cell，2015，14(4)：625-634.
［16］Santos Savio A，Machado Diaz AC，Chico Capote A.Differential expression of pro-inflammatory cytokines IL-15Rαlpha，IL-15，IL-6 and TNFalpha in synovial fluid from rheumatoid arthritispatients［J］.BMC Musculoskelet Disord，2015，12(5)：16-51.
［17］Petrovic-Rackov L，Pejnovic N.Clinical significance of IL-18，IL-15，IL-12 and TNF-alpha measurement in rheumatoid arthritis［J］.Clin Rheumatol，2006，25(4)：448-452.
［18］Loro E，Ramaswamy G，Chandra A.IL-15Rα is required for osteoblast function and bone mineralization［J］.Bone，2017，103(10)：20-30.
［19］Kim DH，Jeong YS，Chon J，et al.Association between interleukin 15 receptor，alpha(IL15RA)polymorphism and Korean patients with ossification of the posterior longitudinal ligament［J］.Cytokine，2011，55(3)：343-348.
［20］Guo Q，Lv SZ，Wu SW，et al.Association between single nucleotide polymorphism of IL15RA gene with susceptibility to ossification of the posterior longitudinal ligament of the spine［J］ J Orthop Surg Res，2014(9)：103.