miR-34a在骨肉瘤患者血清中的表达及在诊断和预后中的价值

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Abstract Objective To investigate the expression and clinical significance of circulation miR-34a in patients with osteoscarma(OS).Methods 54 patients with OS and 54 healthy peoples from Hanchuan City People’ Hospital were prospectively analysed.There were 23 males and 31 females with an average age of (15.6±2.3) years (10~30 years).There were 25 males and 29 females with an average age of (16.3±2.1) years (range：10~30 years).The expression of miR-34a in serum of all the people was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR)，receiver operating characteristic curves (ROC) was used to determine the diagnostic value of OS.The relationship between the miRNA-34a and clinicopathological parameters of OS was single factor and multiple factor analyzed.The relationship between the expression of miR-34a and the survival rate of patients with OS was analysed by Kaplan Meier and log rank test. Results The level of miR-34a in serum of patients with OS was significantly lower than the healthy control (P＜0.01).ROC showed that the AUC was 0.830，the sensitivity and specificity were 79.6% and 75.9% respectively.The expression of miR-34a was related to tumor size，TNM stage and metastasis (P＜0.05)，but not to gender，age and tumor location (P＞0.05).The TNM stage and metastasis were the independent factor influencing the expression of miR-34a.The overall survival rate of patients with the low expression of miR-34a was significantly lower than that with high expression (P＝0.003).Conclusion miR-34a with the tumor suppressor gene may be involved in the genesis，development and prognosis of OS，and may be a potential serum molecular marker for early diagnosis of OS.

Key words： osteoscarma miR-34a molecular marker diagnosis prognosis

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	Luo Lang
	He Wei
	Zhang Lei *

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Luo Lang,He Wei,Zhang Lei *. Expression of Serum miR-34a in Patients with Osteoscarma and Its Value in Diagnosis and Prognosis[J]. sygkzz, 2021, 27(3): 224-234.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2021/V27/I3/224

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