lncRNA FGD5-AS1调控miR-761影响骨肉瘤细胞增殖、迁移和凋亡的机制研究

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Abstract Objective To investigate the effect of long-chain non-coding RNA(lncRNA) PH domain containing 5 antisense RNA 1(FGD5-AS1) on the proliferation，migration and apoptosis of osteosarcoma cells by targeting miR-761.Methods Osteosarcoma tissue and paratumoral tissue samples were collected from 30 patients with osteosarcoma，who underwent surgery in our hospital from 2016 to 2018.There were male 18 and female 12，age 7~69 years old，mean age (20.2±1.29) years old.The expression level of FGD5-AS1 was detected by real-time fluorescent quantitative PCR (RT-qPCR).Osteosarcoma cells U2OS were divided into transfection small interfering RNA negative control (si-NC) group，transfection of si-FGD5-AS1 (si-FGD5-AS1) group，transfection of si-FGD5-AS1 and miRNA mimics negative control (si-FGD5-AS1+miR-NC) group，transfection of si-FGD5-AS1 plus miR-761 mimics (si-FGD5-AS1+miR-761) group，transfection of si-FGD5-AS1 plusmiRNA inhibitor negative control (si-FGD5-AS1+anti-miR-NC) group and transfection of si-FGD5-AS1 plus miR-761 inhibitors (si-FGD5-AS1+anti-miR-761) group.The methyl thiazolyl tetrazolium (MTT) method，colony formation experiment，and flow cytometry were used to detect cell viability，colony forming ability，and apoptosis，respectively.Double luciferase reporting experiments and RT-qPCR confirmed the targeted regulation of FGD5-AS1 on miR-761.Results Compared with adjacent tumor tissues，the expression level of FGD5-AS1 in osteosarcoma tissues was significantly increased (P＜0.05).Compared with the si-NC group，the survival rate，clone formation numbers，migrating cell numbers of U2OS cells in the si-FGD5-AS1 group was significantly reduced，while cell apoptosis rate was significantly increased (P＜0.05).Compared with the si-FGD5-AS1+miR-NC group，the survival rate，clone formation numbers，migrating cell numbers of U2OS cells in the si-FGD5-AS1+miR-761 group was significantly reduced，while cell apoptosis rate was significantly increased (P＜0.05).Compared with the si-FGD5-AS1+anti-miR-NC group，the survival rate，clone formation numbers，migrating cell numbers of U2OS cells in the si-FGD5-AS1+anti-miR-761 group was significantly increased，while cell apoptosis rate was significantly reduced (P＜0.05).FGD5-AS1 has a targeted negative regulatory effect on miR-761.Conclusion FGD5-AS1 is highly expressed in osteosarcoma.Inhibiting FGD5-AS1 inhibits the proliferation and migration，and induce apoptosis of osteosarcoma cells by up-regulating miR-761.

Key words： FGD5-AS1 miR-761 osteosarcoma proliferation migration apoptosis

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	Li Feng
	Gong Jicheng
	Du Jingzhu
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Li Feng,Gong Jicheng,Du Jingzhu, et al. FGD5-AS1 Regulating miR-761 Affecting the Proliferation，Migration and Apoptosis of Osteosarcoma Cells[J]. sygkzz, 2021, 27(3): 228-234.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2021/V27/I3/228

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