miRNA-27α调控PPARγ/ApoA5通路对大鼠激素性股骨坏死的影响研究

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Abstract Objective To investigate the effect of miRNA-27α regulating peroxisome proliferator-activated receptor (PPARγ)/apolipoprotein A5(ApoA5) pathway on steroid-induced femoral head necrosis in rats induced by methylprednisolone.Methods Thirty female SPF-grade SD rats were divided into 5 groups according to the random number method，with 6 in each group.1 group was a blank control group；4 groups were model groups.An in vitro model of femoral head necrosis was induced by methylprednisolone.Immunohistochemical analysis of bone morphology，qRT-PCR analysis of gene expression changes，and MTT method were used to detect cell proliferation and viability.Results Hormone-induced rat femoral head sections showed sparse，broken，and disordered bone trabeculae，significantly reduced the area of bone trabeculae，and significantly reduced the content of miRNA-27α (P＜0.05).The levels of PPARγ and ApoA5 mRNA expression were significant increased (P＜0.05).PPARγ and ApoA5 showed a negative correlation with miRNA-27α，with correlation coefficients of 0.725 and 0.683，respectively.In the injection group of methylprednisolone and miRNA-27α mimics，the levels of PPARγ and ApoA5 mRNA significantly decreased (P＜0.05)；the proliferation of miRNA-27α mimics group was significantly higher than that of miRNA-27α inhibitor group.The chondrocyte survival rate in the model group was significantly reduced，but the miRNA-27α inhibitor group also produced a greater inhibitory effect.Conclusion miRNA-27α can delay the progress of hormonal femoral head necrosis by inhibiting the expression of PPARγ / ApoA5.

Key words： miRNA-27α peroxisome proliferator-activated receptor apolipoprotein A5 steroid-induced osteonecrosis

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	Huang Tao
	Cui Yong *

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Huang Tao,Cui Yong *. Effect of miRNA-27α regulating PPARγ/ApoA5 pathway on hormonal femoral necrosis in rats[J]. sygkzz, 2021, 27(4): 330-334.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2021/V27/I4/330

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