MiR-367通过Wnt/β-catenin通路抑制成骨细胞增殖的研究

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Abstract Objective To study the inhibitory effect of MiR-367 on osteoblast proliferation through Wnt/β-catenin pathway.Methods Bone marrow mesenchymal stem cells (BMSCs) were transfected with mimic or inhibitor of miR-367，and osteogenic differentiation was observed by alizarin red staining.Osteoblasts from neonatal rats were cultured and transfected with mimic or inhibitor of miR367，combined with SKL2001 or XAV939 as an agonist or antagonist of β-catenin.Cell proliferation activity was measured by MTT method，and the expression of β-catenin and osteogenic markers gene RUNX2，ALP，OCN，Col-Ⅰ were detected by fluorescence quantitative PCR and Western blot.Results After transfection of mir367 mimics or inhibitor，OD₅₇₀ value of alizarin red staining had no significant difference with those transfected with NC mimic or inhibitor(P＞0.05).OD₄₉₀ value of MTS and expression levels of β-catenin，RUNX2，ALP，OCN and Col-Ⅰ in osteoblasts of neonatal rats after transfection of miR-367 mimic were significantly lower than those of NC mimic，OD₄₉₀value of MTS and expression levels of β-catenin，RUNX2，ALP，OCN and Col-Ⅰ in osteoblasts of neonatal rats after transfection of mir-367 inhibitor were significantly higher than those of NC inhibitor(P＜0.05).The expression levels of β-catenin，RUNX2，ALP，OCN and Col-Ⅰ in osteoblasts of newborn rats treated with miR-367 mimic combined with SKL2001 were significantly higher than those of miR-367mimic.The expression levels of β-catenin，RUNX2，ALP，OCN and Col-Ⅰ in osteoblasts of newborn rats treated with miR-367 inhibitor combined with SKL2001 were significantly lower than those of miR-367 inhibitor.Conclusion MiR-367 can inhibit the proliferation of osteoblasts，but does not affect the differentiation of osteoblasts，which may be achieved by inhibiting Wnt/β-catenin pathway.

Key words： osteoblasts cell proliferation cell differentiation miR-367 Wnt/β-catenin pathway

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	Ma Jianguo
	Gao Zhiming
	Jin Lei *

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Ma Jianguo,Gao Zhiming,Jin Lei *. MiR-367 Inhibits Osteoblast Proliferation through Wnt/β-catenin Pathway[J]. sygkzz, 2020, 26(1): 34-39.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2020/V26/I1/34

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