阿仑膦酸钠、氟化钠联合作用对体外培养大鼠成骨细胞的影响

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Abstract Objective To study the effect of alendronate combined with sodium fluoride on osteoblasts in vitro.Methods To observe the proliferation and alkaline phosphatase(ALP) activity，the osteoblasts primarily cultured in vitro were isolated from the skull of rats by trypsin digestion method，andstimulated by alendronate and sodium fluoride at different concentrations.The expression of OPG/RANKL was examined by Western Blotting.Results There was the most significant promotion in osteoblast differentiation and proliferation when the osteoblasts were cultured at the concentration of alendronate of 10^－6mmol/L and sodium fluoride of 10^－8mmol/L.Compared with the control group，the osteoblastsincombined therapy group had significantly promotion in proliferation and ALP activity.The expression of OPG was increased，and the expression of RANKL was decreased.There were statistically significantdifferences in proliferation，ALP activity and expression of OPG/RANKL.An inhibitory effect was observed at high concentrations of sodium fluoride (10^－3mmol/L、10^－4mmol/L ).Conclusion Both alendronate and sodium fluoride can promote osteoblast proliferation and differentiation，and there is synergistic effect when they combined.Sodium fluoride at high concentrations had an inhibitory effect on osteoblasts.

Key words： alendronate sodium fluoride osteoblasts proliferation

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