|
|
|
Abstract Objective To investigate the effect of lidocaine on anabolism of cultured rabbit knee articular chondrocytes.Methods Six anesthetized New Zealand big white rabbits were killed.We collected knee cartilage and separated articular chondrocytes，which were exposed to 2 mmoL//L lidocaine or saline for 3﹑6 or 9 days.The relative expression of Collagen Ⅱ and aggrecan of articular chondrocytes were assessed with RT-RNA.SPSS 13.0 software was used to analyze the data.Results RT-PCR assay showed the relative expression of Collagen Ⅱ mRNA exposed to 2 mmoL/L lidocaine for 3﹑6 or 9 days was (18.51±2.34)﹑(16.95±3.10)﹑(15.79±3.57)，the ones exposed to saline for 3﹑6 or 9 days was (19.48±4.01)(18.76±1.96)(17.81±3.05)respevtively，there wasn’t statistically significant difference between lidocaine groups and saline groups (t＝－0.32﹑－0.45﹑－0.72)(All -P＞0.05).The relative expression of aggrecan mRNA exposed to 2mmoL/L lidocaine for 3﹑6 or 9 days was(9.72±0.32)﹑(9.59±0.78)﹑(12.54±1.06)，the ones exposed to saline for 3﹑6 or 9 days was (10.68±1.07)﹑(10.06±0.93)﹑(9.87±1.12)respevtively，there was statistically significant difference between lidocaine and saline groups at the ninth day (t＝2.59，P＜0.05).Conclusion The effect was limited on anabolism of cultured rabbit knee articular chondrocytes exposed to lidocaine at finite time and concentration.
|
|
|
|
|
|
［1］Hansen BP，Beck CL，Beck EP，et al.Postarthoroscopic glenohumeral chondrolysis［J］.Am J Sports Med，2007，35(10)：1628-1634.
［2］杨朝晖.钠离子通道阻断剂利多卡因体内外干预关节软骨的生物学效应［D］.太原：山西医科大学，2011.
［3］卫小春.关节软骨［M］.北京：科学出版社，2007：3-4.
［4］Sasaki T，Watanabe C.Stimulation of osteoinduction in bone wound healing by high-molecular hyaluronic acid［J］.Bone，1995，16 (1)：9-15.
［5］徐莉，王建.关节腔内注入罗哌卡因-利多卡因和芬太尼用于膝关节镜术后镇痛的临床研究［D］.成都：四川大学，2006.
［6］Hille B.The pH dependent rate of action of local anesthetics of the node of Ranvier［J］.J Gen Physiol，1977，69(4)：475-496.
［7］Togo T，Utani A，Naitoh M，et al.Identification of cartilage progenitor cells in the adult ear perichondrium：utilization for cartilage reconstruction［J］.Laboratory Investigation，2006，86(5)：445-457.
［8］Kim DK，Jankowski RJ，Druchnic R，et al.In vitro and in vivo effect of lodocaine on rat muscle derived cells for treatment of stress urinary incontinence［J］.Urology，2009，73(2)：437-441.
［9］Wohlrab D，Lebek S，Krüger T，et al.Influence of ion channels on the proliferation of human chondrocytes［J］.Biorheology，2002，39(1-2)：55-61.
［10］Takeno K，Kobayashi S，Miyazaki T，et al.Lidocaine cytotoxicity to the zygapophysial joints in rabbits［J］.Spine，2009，34(26)：945-951. |
|
|
���������� 14010702070875��