RANKL/RANK/OPG信号通路参与调控磷酸三钙磨损颗粒诱导假体周围骨溶解的研究

摘要
图/表
参考文献
相关文章 (1)

全文: PDF (1482 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的探讨RANKL/RANK/OPG信号通路在磷酸三钙磨损颗粒诱导假体周围骨溶解中的作用。方法将30只小鼠随机分为假手术组、模型组和OPG组，制备小鼠颅骨溶解模型，OPG组于术后第2天在颅顶局部注射骨保护素(osteoprotegerin，OPG)(3 mg/kg)，每隔2 d 1次；假手术组和模型组给予等量生理盐水，共2周。测定破骨细胞数和骨溶解面积，检测白细胞介素-1β(Interleukin-1β，IL-1β)、白细胞介素-6(Interleukin-6，IL-6)和肿瘤坏死因子-α(tumor necrosis factor α，TNF-α)、OPG、NF-kappa B的受体激活因子(receptor activator of nuclear factor-κB，RANK)和RANK配体(receptor activator of nuclear factor-κB ligand，RANKL)的水平。结果与假手术组比较，模型组小鼠颅骨破骨细胞数、骨溶解面积均增加，IL-1β、IL-6和TNF-α水平均增加，RANKL和RANK mRNA的相对表达量均增加，OPG mRNA的相对表达量降低(P＜0.05)；与模型组比较，OPG组小鼠颅骨破骨细胞数和骨溶解面积减少，IL-1β、IL-6和TNF-α水平减少，RANKL和RANK mRNA的相对表达量均降低，OPG mRNA的相对表达量增加(P＜0.05)。结论 RANKL/RANK/OPG信号通路参与调控磷酸三钙磨损颗粒诱导假体周围骨溶解，通过给予外源性OPG能显著抑制磷酸三钙磨损颗粒诱导的骨溶解。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章

关键词 ： RANKL/RANK/OPG信号通路, 磷酸三钙磨损颗粒, 骨溶解

Abstract：Objective To investigate the role of RANKL/RANK/OPG signaling pathway in periprosthetic osteolysis induced by tricalcium phosphate wear particles.Methods 30 mice were randomly divided into sham operation group，model group and OPG group.The murine calvarial model of osteolysis mice were induced by tricalcium phosphate wear particles.OPG (3mg/kg) was locally injected to the calvarium under the periosteum every two weeks，while the sham operation group and the model group were given the same amount of saline.2 weeks later，the number of osteoclasts and osteolysis area，the levels of IL-1β，IL-6 and TNF-α in the periosteum of the skull and the mRNA expression levels of RANKL，RANK and OPG in the skull were measured.Results Compared with the sham-operated group，the number of osteoclasts and osteolysis area in the model group and OPG group increased，the levels of IL-1β，IL-6 and TNF-α in the periosteum increased，the relative expression of RANKL mRNA and RANK mRNA in the skull increased，and the relative expression of OPG mRNA decreased (P<0.05).Compared with the model group，the number of osteoclasts and osteolysis area in the skull of OPG group decreased.The levels of IL-1β，IL-6 and TNF-α in periosteum decreased，the relative expression of RANKL mRNA and RANK mRNA in skull decreased，and the relative expression of OPG mRNA increased (P<0.05).Conclusion RANKL/RANK/OPG signaling pathway is involved in the regulation of tricalcium phosphate wear particles induced periprosthetic osteolysis，while the OPG intervention can significantly inhibit osteolysis，which can be used as a therapeutic target for periprosthetic osteolysis.

Key words： RANKL/RANK/OPG signaling pathway tricalcium phosphate wear particles osteolysis

基金资助:国家自然科学基金资助项目(81702214)

通讯作者: 余勤武

作者简介: 贺志强(1983－ )，男，主治医师，仙桃市第一人民医院骨外一科，433000。

引用本文:

贺志强 1，杨亚帆 1，陈先州 1，刘乃澄 2，余勤武 1*. RANKL/RANK/OPG信号通路参与调控磷酸三钙磨损颗粒诱导假体周围骨溶解的研究[J]. 实用骨科杂志, 2019, 25(11): 991-995.
He Zhiqiang，Yang Yafan，Chen Xianzhou，et al. Role of RANKL/RANK/OPG Signaling Pathway in Periprosthetic Osteolysis Induced by Tricalcium Phosphate Wear Particles. sygkzz, 2019, 25(11): 991-995.

链接本文:

http://www.sygkzz.com/CN/ 或 http://www.sygkzz.com/CN/Y2019/V25/I11/991

［1］韩以杰，魏招阳，李智波，等.半髋关节置换和锁定钢板内固定治疗老年股骨转子间骨折的疗效［J］.实用骨科杂志，2016，22(6)：544-547.
［2］Viste A，Zahrani NA，Brito N，et al.Reply to the letter to the editor：Periprosthetic osteolysis after AES total ankle replacement：Conventional radiography versus CT scan［J］.Foot Ankle Surg，2016，22(2)：143.
［3］Van TD，Tran NQ，Nguyen DH，et al.Injectable hydrogel composite based gelatin-PEG and biphasic calcium phosphate nanoparticles for bone regeneration［J］.J Elec Materi，2016，45(5)：2415-2422.
［4］Chapurlat RD，Confavreux CB.Novel biological markers of bone：From bone metabolism to bone physiologya［J］.Rheumatology，2016，55(10)：1714-1725.
［5］Wolski H，Drews K，Bogacz A，et al.The RANKL/RANK/OPG signal trail：significance of genetic polymorphisms in the etiology of postmenopausal osteoporosis［J］.Ginekologia Polska，2016，87(5)：347-352.
［6］Jihai W，Xuejian W，Yongzhuang D.Magnesium lithospermate B protects against lipopolysaccharide-induced bone loss by inhibiting RANKL/RANK pathway［J］.Front Pharmacol，2018，9(3)：64-72.
［7］Varley I，Hughes DC，Greeves JP，et al.SNPs in the vicinity of P2X7R，RANK/RANKL/OPG and Wnt signalling pathways and their association with bone phenotypes in academy footballers［J］.Bone，2018，108(14)：179-185.
［8］Qin S，Zhang Q，Zhang L.Effect of OPG gene mutation on protein expression and biological activity in osteoporosis［J］.Exp Ther Med，2017，14(2)：1475-1480.
［9］李金龙，周凯，陈志，等.大直径股骨头金属对金属人工全髋关节置换术后中远期疗效评价及失败原因分析［J］.中国修复重建外科杂志，2017，16(2)：144-149.
［10］Naranjo M C，Garcia I，Bermudez B，et al.Acute effects of dietary fatty acids on osteclastogenesis via RANKL/RANK/OPG system［J］.Mol Nutr Food Res，2016，60(11)：2505-2513.
［11］Ormsby RT，Cantley M，Kogawa M，et al.Evidence that osteocyte perilacunar remodelling contributes to polyethylene wear particle induced osteolysis［J］.Acta Biomater，2016，33(9)：242-251.
［12］Park JH，Lee NK，Lee SY.Current understanding of RANK signaling in osteoclast differentiation and maturation［J］.Molecules & Cells，2017，40(10)：706-713.
［13］Xiao Y，Palomero J，Grabowska J，et al.Macrophages and osteoclasts stem from a bipotent progenitor downstream of a macrophage/osteoclast/dendritic cell progenitor［J］.Blood Advances，2017，1(23)：1993-2006.
［14］Ganesan R，Rasool M.Interleukin 17 regulates SHP-2 and IL-17RA/STAT-3 dependent Cyr61，IL-23 and GM-CSF expression and RANKL mediated osteoclastogenesis by fibroblast-like synoviocytes in rheumatoid arthritis［J］.Molecular Immunology，2017，91(12)：134-144.
［15］Xu S，Zhang Y，Liu B，et al.Activation of mTORC1 in B lymphocytes promotes osteoclast formation via regulation of β-Catenin and RANKL/OPG［J］.J Bone Miner Res，2016，31(7)：1320-1333.
［16］Elizabeth K，Peter G，Xiaodong J，et al.The Effect of RANKL/OPG balance on reducing implant complications［J］.J Funct Biomater，2017，8(4)：42-49.
［17］Tao C，Yaochao Z，Bin L，et al.Curcumin attenuation of wear particle-induced osteolysis via RANKL signaling pathway suppression in mouse calvarial model［J］.Mediators Inflamm，2017，12(5)：1-11.
［18］Chaganti RK，Purdue E，Sculco TP，et al.Elevation of serum tumor necrosis factor α in patients with periprosthetic osteolysis：a case-control study［J］.Clin Orthop Relat Res，2014，472(2)：584-892.
［19］刘国印，王瑞，董磊，等.内质网应激介导成骨细胞凋亡在骨溶解骨组织中的作用及机制［J］.中国组织工程研究，2014，18(33)：5257-5265.