PLGA导管复合骨髓间充质干细胞与细胞外基质凝胶移植修复大鼠坐骨神经缺损的生物力学分析

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摘要目的研究坐骨神经损伤动物模型以聚乳酸羟基乙酸共聚物［poly(lactic-co-glycolic acid)，PLGA］导管复合骨髓间充质干细胞(bone mesenchymal stem cells，BMSCs)与细胞外基质(extracellular matrix，ECM)凝胶移植后坐骨神经的生物力学特性，为临床提供生物力学等基础研究支持。方法大鼠坐骨神经损伤模型，分别以自体神经、PLGA导管复合BMSCs、PLGA导管复合BMSCs与ECM凝胶移植6、12、18、24周，对各组大鼠进行大体行为学观察，各组大鼠移植168 d时进行坐骨神经功能指数测量，之后取各组大鼠坐骨神经进行拉伸力学性能实验。结果 PLGA导管复合BMSCs移植组、PLGA导管复合BMSCs与ECM凝胶移植组，在拉伸弹性限度载荷、弹性限应力、最大载荷、最大应力、最大应变、弹性限度应变均大于自体神经移植组，差异有统计学意义(P＜0.05)；PLGA导管复合BMSCs与ECM凝胶移植组的拉伸弹性限度载荷、弹性限应力、最大载荷、最大应力、弹性限度应变大于PLGA导管复合BMSCs移植组，差异有统计学意义(P＜0.05)。结论PLGA导管复合BMSCs、PLGA导管复合BMSCs与ECM凝胶移植坐骨神经损伤具有明显的修复效果。

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	王怀程
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	于涛

关键词 ：动物模型, 聚乳酸-羟基乙酸共聚物导管, 骨髓间充质干细胞, 坐骨神经, 损伤

Abstract：Objective To study the animal model of sciatic nerve injury by combining poly［(lactic-co-glycolic acid)，PLGA］catheters with bone marrow mesenchymal stem cells (bone mesenchymal stem cells，BMSCs) combined with extracellular matrix (extracellular matrix，ECM)，biomechanical properties of the sciatic nerve after gel transplantation and provide basic research support for clinical application.Methods The rat sciatic nerve injury model was transplanted with autologous nerve，PLGA catheter combined with BMSCs，PLGA catheter combined with BMSCs and ECM gel for 6，12，18，and 24 weeks，and the general behavior of rats in each group was observed.The functional index of the sciatic nerve was measured 168 days after the transplantation in the rats，and then the sciatic nerves of the rats in each group were taken for tensile mechanical properties experiments.Results The tensile elastic limit load，elastic limit stress，maximum load，maximum stress，maximum strain，and elastic limit strain of the PLGA catheter combined with BMSCs transplantation group and the PLGA catheter combined with BMSCs and ECM gel transplantation group were greater than those of the autologous nerve transplantation group.The difference was statistically significant (P＜0.05).The tensile elastic limit load，elastic limit stress，maximum load，maximum stress，and elastic limit strain of the PLGA catheter composite BMSCs and ECM gel transplantation group were greater than those of the PLGA catheter composite BMSCs transplantation group.The difference was statistically significant (P＜0.05) Conclusion PLGA catheter combined with BMSCs，PLGA catheter combined with BMSCs and ECM gel transplantation have obvious repair effect on sciatic nerve injury.

Key words： animal model poly(lactic-co-glycolic acid) catheter bone marrow mesenchymal stem cells sciatic nerve damage

通讯作者: 于涛

作者简介: 王怀程(1994－ )，男，研究生在读，吉林大学中日联谊医院骨关节科，656194329@qq.com

引用本文:

王怀程，李鹏，汪标文，杜纯宇，于涛. PLGA导管复合骨髓间充质干细胞与细胞外基质凝胶移植修复大鼠坐骨神经缺损的生物力学分析[J]. 实用骨科杂志, 2023, 29(2): 132-.
Wang Huaicheng，Li Peng，Wang Biaowen，Du Chunyu，Yu Tao. Biomechanical Analysis of Plga Catheter Combined With Bmscs and Extracellular Matrix Gel Transplantation for Repairing Sciatic Nerve Defects in Rats. sygkzz, 2023, 29(2): 132-.

链接本文:

http://www.sygkzz.com/CN/ 或 http://www.sygkzz.com/CN/Y2023/V29/I2/132

［1］Liu B，Xin W，Tan JR，et al.Myelin sheath structure and regeneration in peripheral nerve injury repair［J］.Proc Natl Acad Sci USA，2019，(44)：116.
［2］Xu J，Wen J，Fu L，et al.Macrophage-specific RhoA knockout delays Wallerian degeneration after peripheral nerve injury in mice［J］.J Neuroinflammation，2021，18(1)：234.
［3］Li NY，Onor GI，Lemme NJ，et al.Epidemiology of peripheral nerve injuries in sports，exercise，and recreation in the United States，20092018［J］.Phys Sportsmed，2021，49(3)：355-362.
［4］Zhang J，Zhang Y，Chen L，et al.Ulinastatin promotes regeneration of peripheral nerves after sciatic nerve injury by targeting let-7 micrornas and enhancing NGF expression［J］.Drug Des Devel Ther，2020(14)：2695-2705.
［5］季夕萌，庄海，张怡雯，等.载骨髓间充质干细胞双层胶原神经管修复大鼠坐骨神经缺损的研究［J］.中华实验外科杂志，2020，37(1)：4.
［6］龚庆，宋秋莹，邱莉晶，等.PLGA微球搭载鹿茸多肽联合BMSCs移植对坐骨神经损伤修复的作用及机制研究［J］.中药新药与临床药理，2019，30(11)：5.
［7］Vijayavenkataraman S.Nerve guide conduits for peripheral nerve injury repair：A review on design，materials and fabrication methods［J］.Acta Biomater，2020(106)：54-69.
［8］Jo H，Jung M，Seo DJ，et al.The effect of rat bone marrow derived mesenchymal stem cells transplantation for restoration of olfactory disorder［J］.Biochem Biophys Res Commun，2015，467(2)：395-399.
［9］Gattermeyer TJ，Hanson SC，Rodgers JR.System comprising a tool for decoupling cross-fire tube assemblies in gas turbine engines［P］.EP3306198B1，2020-04.
［10］Quirk RA，Chan WC，Davies MC，et al.Poly(L-lysine)-GRGDS as a biomimetic surface modifier for poly(lactic acid)［J］.Biomaterials，2001，22(8)：865-872.
［11］Shen H，Hu X，Yang F，et al.Combining oxygen plasma treatment with anchorage of cationized gelatin for enhancing cell affinity of poly(lactide-co-glycolide)［J］.Biomaterials，2007，28(29)：4219-4230.
［12］Dolder J，Farber E，Spauwen P，et al.Bone tissue reconstruction using titanium fiber mesh combined with rat bone marrow stromal cells［J］.Biomaterials，2003，24(10)：1745-1750.
［13］张学磊，罗干，于圣会，等.脱细胞神经联合骨髓间充质干细胞及富血小板凝胶治疗股神经损伤［J］.中国组织工程研究，2022，26(1)：6.
［14］张威，魏雅楠，韩娜.骨髓间充质干细胞来源外泌体对促进大鼠坐骨神经钳夹伤的修复作用［J］.中华临床医师杂志：电子版，2021，15(4)：7.
［15］Pereira Lopes FR，Camargo de Moura Campos L，Dias Corrêa J Jr，et al.Bone marrow stromal cells and resorbable collagen guidance tubes enhance sciatic nerve regeneration in mice［J］.Exp Neurol，2006，198(2)：457-468.
［16］Yu T，Zhao C，Li P，et al.Poly(lactic-co-glycolic acid) conduit for repair of injured sciatic nerve A mechanical analysis［J］.Neural Regen Res，2013，8(21)：1966-1973.
［17］Lopes FP，Langone F，Balduino A，et al.Bone marrow stromal cells and resorbable collagen guidance tubes enhance sciatic nerve regeneration in mice［J］.Exp Neurol，2006，198(2)：457-468.
［18］Baptista AF，Gomes JR，Oliveira JT，et al.A new approach to assess function after sciatic nerve lesion in the mouse—Adaptation of the sciatic static index［J］.J Neurosci Methods，2007，161(2)：259-264.
［19］Piao C，Li Z，Ding J，et al.Analysis of BMSCs-intervened viscoelasticity of sciatic nerve in rats with chronic alcoholic intoxication 1［J］.Acta Cir Bras，2018，33(10)：935-944.
［20］Piao C，Li Z，Ding J，et al.Mechanical properties of the sciatic nerve following combined transplantation of analytically extracted acellular allogeneic nerve and adipose-derived mesenchymal stem cells［J］.Acta Cir Bras，2020，35(4)：e202000405.