miR-140-3p靶向凋亡基因BAX减轻白细胞介素-1β诱导的软骨细胞损伤

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摘要目的研究微小RNA(microRNA，miR)-140-3p靶向凋亡基因Bcl-2相关X蛋白(Bcl-2 associatal X protein，BAX)减轻白细胞介素(interleukin，IL)-1β诱导软骨细胞损伤的作用及机制。方法培养大鼠骨关节软骨细胞ATDC5，采用不同浓度IL-1β(0、1、5、10ng/mL)处理后检测miR-140-3p、BAX的表达水平；10 ng/mL IL-1β处理的同时转染阴性对照(NC)miR或miR-140-3p、感染NC腺病毒或BAX腺病毒，检测细胞存活率、凋亡率、miR-140-3p、BAX及Cleaved caspase-3表达水平；采用双荧光素酶报告基因实验验证miR-140-3p靶向BAX的作用。结果不同浓度IL-1β处理的ATDC5细胞中miR-140-3p表达降低、BAX表达增加(P＜0.05)；miR-NC+IL-1β组的存活率、miR-140-3p表达水平低于miR-NC组，凋亡率、BAX及Cleaved caspase-3表达水平高于miR-NC组(P＜0.05)；miR-140+IL-1β组的存活率、miR-140-3p表达水平高于miR-NC+IL-1β组，凋亡率、BAX及Cleaved caspase-3表达水平低于miR-NC+IL-1β组(P＜0.05)；miR-140+Ad-BAX+IL-1β组的存活率低于miR-140+Ad-NC+IL-1β组，凋亡率、BAX及Cleaved caspase-3表达水平高于miR-140+Ad-NC+IL-1β组(P＜0.05)。结论在IL-1β诱导软骨细胞损伤模型中miR-140-3p起到保护作用，靶向BAX并抑制细胞凋亡是相关的分子机制。

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	王燚伟
	田永利 *
	马广恩
	张永占

关键词 ：骨关节炎, 白细胞介素-1β, 微小RNA-140-3p, Bcl-2相关X蛋白, 凋亡

Abstract：Objective To study the effect and mechanism of miR-140-3p in attenuating interleukin-1 β(IL-1β) induced chondrocyte injury via targeting apoptosis gene Bcl-2 associated X protein (BAX).Methods Rat osteoarticular chondrocytes ATDC5 were cultured and the expression levels of miR-140-3p and BAX were detected after the treatment of different concentration of IL-1β(0，1，5，10ng/mL).Then cells were treated with 10ng/mL IL-1β，at the same time，negative control (NC) miR or miR-140-3p was transfected，NC adenovirus or BAX adenovirus was infected.The cell survival rate，apoptosis rate，miR-140-3p，Bax and cleaved caspase-3 expression levels were detected.The dual luciferase reporter gene experiment was used to verify the effect of miR-140-3p targeting BAX.Results The expression of miR-140-3p decreased and BAX increased in ATDC5 cells treated with different concentrations of IL-1 β(P＜0.05).The survival rate and the expression level of miR-NC+IL-1β group were lower than those of miR-NC group，while the apoptosis rate and the expression levels of BAX and Cleaved caspase-3 were higher than those of miR-NC group(P＜0.05).The survival rate and the expression level of miR-140+IL-1β group were higher than those of miR-NC+IL-1β group，while the apoptosis rate and the expression levels of BAX and Cleaved caspase-3 were lower than those of miR-NC+IL-1β group(P＜0.05).The survival rate of miR-140+Ad-BAX+IL-1β group was lower than that of miR-140+Ad-NC+IL-1β group，while the apoptosis rate and the expression levels of BAX and Cleaved caspase-3 were higher than those of miR-140+Ad-NC+IL-1β group(P＜0.05).Conclusion IL-1β plays a protective role in IL-1β induced chondrocyte injury model，and targeting BAX and inhibiting apoptosis are related molecular mechanisms.

Key words： osteoarthritis interleukin-1β miR-140-3p Bcl-2 related X protein apoptosis

通讯作者: 田永利

作者简介: 王燚伟(1984－ )，男，主治医师，秦皇岛市第一医院关节骨科，066001。

引用本文:

王燚伟，田永利 *，马广恩，张永占. miR-140-3p靶向凋亡基因BAX减轻白细胞介素-1β诱导的软骨细胞损伤[J]. 实用骨科杂志, 2021, 27(12): 1102-1116.
Wang Yiwei，Tian Yongli *，Ma Guangen，et al. MiR-140-3p Attenuates Interleukin-1 β Induced Chondrocyte Injury Via Targeting Apoptosis Gene BAX. sygkzz, 2021, 27(12): 1102-1116.

链接本文:

http://www.sygkzz.com/CN/ 或 http://www.sygkzz.com/CN/Y2021/V27/I12/1102

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