Orai2对成骨细胞增殖、凋亡及分化的作用研究

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Abstract Objective To determine the role of Orai2 in regulating osteoblast proliferation，apoptosis and differentiation in osteoblasts.Methods Western blot and real-time PCR were used to detect the protein expression and mRNA transcription levels of Orai2 after osteoblastic induction.The expression of Orai2 was silenced in MC3T3-E1 osteoblasts by transfecting Orai2 shRNA，and unrelated interference shRNA was transfected as negative control.MC3T3-E1 osteoblasts were divided into control group and Orai2 shRNA group.In the control group and Orai2 shRNA group，cell proliferation and apoptosis，the transcription of Runx2，Osterix and ALP，and Ras-ERK1/2 signaling pathway activity were tested to dentify whether Orai2 is involved in the regulation of osteogenesis.Results After osteogenic induction，Orai2 protein expression level and mRNA transcription level were gradually increased (P＜0.05).Compared with the control group，Orai2 was knockdown successfully after Orai2 shRNA transfected in MC3T3-E1 cells.Furthermore，compared with the control group，the proliferation were decreased and apoptosis were increased after Orai2 knockdown.Moreover，compared with the control group，the transcription of Runx2，Osterix and ALP，were decreased after Orai2 knockdown.We also found that Ras-ERK1/2 signaling pathway activity were inhibited compared with the control group.Conclusion The inhibition of Orai2 expression can significantly reduce the activity of Ras-Erk1/2 signaling pathway，reduce the proliferation of osteoblasts，increase the apoptosis of osteoblasts，and inhibit the differentiation of osteoblasts，resulting in the decrease in the number of osteoblasts and the involvement in the occurrence and development of osteoporosis.

Key words： Orai2 proliferation apoptosis differentiation osteoblast

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