基质金属蛋白酶-11在骨肉瘤中的表达及生物学功能研究

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Abstract Objective To detect the expression of matrix met alloproteinases 11 (MMP-11) in osteosarcoma tissues and adjacent matched normal osteosarcoma tissues，and explore the effect of silencing MMP-11 expression on the biological behaviors of osteosarcoma cells.Methods MMP-11 expression was detected in 40 paired osteosarcoma tissues and adjacent matched normal osteosarcoma tissues using Quantitative RT-PCR analysis and immunohistochemistry technology.MMP-11 siRNA and siRNA control were transfected into osteosarcoma cells (HOS and Saos-2).RNA and protein were collected after 48 h，and Quantitative RT-PCR analysis and Western blots were applied to detected the expression of MMP-11 in osteosarcoma cells.3-(4，5-dimethyl-2-thiazolyl)-2，5-diphenyl-2-H-tetrazolium bromide (MTT)，wound-healing assay and flow cytometry were applied to detected the growth，migration and apoptosis of osteosarcoma cells after RNAi-mediated silencing of MMP-11，respectively.Results The MMP-11 mRNA expression in osteosarcoma tissues was significantly up regulated than adjacent matched normal osteosarcoma tissues (P＜0.05).The mRNA expression levels of MMP-11 in the adjacent matched normal osteosarcoma tissues was (1.0±0.22)，while its relative expression in osteosarcoma tissues was (3.94±0.79)(P＜0.05).Immunohistochemical technique also showed that the positive rate of MMP-11 protein in osteosarcoma was significantly higher than that of in the adjacent matched normal osteosarcoma tissues (P＜0.05).Transfection of MMP-11 siRNA in osteosarcoma cells significantly reduced the expression levels of MMP-11 (P＜0.05).Knockdown of MMP-11 markedly inhibited cell growth and migration，and promoted cell apoptosis (P＜0.05).Conclusion MMP-11 is high expression in osteosarcoma.Knockdown of MMP-11 inhibits cell proliferation and migration，and induces cell apoptosis in osteosarcoma cells，suggesting MMP-11 acts as an oncogene involved in the development of osteosarcoma.

Key words： osteosarcoma matrix met alloproteinase-11 high expression proliferation migration apoptosis

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［1］Siegel RL，Miller KD，Jemal A.Cancer Statistics，2016［J］.CA Cancer J Clin，2016，66(1)：7-30.
［2］Picci P.Osteosarcoma (Osteogenic Sarcoma)［J］.Orphanet J Rare Dis，2007(2)：6.
［3］Mirabello L，Troisi RJ，Savage SA.Osteosarcoma incidence and survival rates from 1973 to 2004：Data from the surveillance，epidemiology，and end results program［J］.Cancer，2009，115(7)：1531-1543.
［4］Pittayapruek P，Meephansan J，Prapapan O，et al.Role of matrix met alloproteinases in photoaging and photocarcinogenesis［J］.Int J Mol Sci，2016，17(6)：868.
［5］Li WM，Wei YC，Huang CN，et al.Matrix Met alloproteinase-11 as a Marker of Metastasis and Predictor of Poor Survival in Urothelial Carcinomas［J］.J Surg Oncol，2016，113(6)：700-707.
［6］Nonsrijun N，Mitchai J，Brown K，et al.Overexpression of matrix met alloproteinase 11 in thai prostatic adenocarcinoma is associated with poor survival［J］.Asian Pac J Cancer Prev，2013，14(5)：3331-3335.
［7］Yan D，Dai H，Liu JW.Serum levels of Mmp-11 correlate with clinical outcome in Chinese patients with advanced gastric adenocarcinoma［J］.BMC Cancer，2011(11)：151.
［8］Hsin CH，Chou YE，Yang SF，et al.Mmp-11 promoted the oral cancer migration and Fak/Src activation［J］.Oncotarget，2017，8(20)：32783-32793.
［9］Iwasaki M，Zhao H，Jaffer T，et al.Volatile anaesthetics enhance the metastasis related cellular signalling including Cxcr2 of ovarian Cancer cells［J］.Oncotarget，2016，7(18)：26042-26056.
［10］Piperigkou Z，Manou D，Karamanou K，et al.Strategies to target matrix met alloproteinases as therapeutic approach in cancer［J］.Methods Mol Biol，2018(1731)：325-348.
［11］Naim A，Pan Q，Baig MS.Matrix met alloproteinases (Mmps) in liver diseases［J］.J Clin Exp Hepatol，2017，7(4)：367-372.
［12］Johnson JL.Met alloproteinases in atherosclerosis［J］.Eur J Pharmacol，2017(816)：93-106.
［13］Boziki M，Grigoriadis N.An update on the role of matrix met alloproteinases in the pathogenesis of multiple sclerosis［J］.Med Chem，2018，14(2)：155-169.
［14］Almendro V，Ametller E，Garcia-Recio S，et al.The role of Mmp7 and its cross-talk with the fas/fasl system during the acquisition of chemoresistance to oxaliplatin［J］.PLoS One，2009，4(3)：e4728.
［15］Li H，Qiu Z，Li F，et al.The Relationship between Mmp-2 and Mmp-9 expression levels with breast cancer incidence and prognosis［J］.Oncol Lett，2017，14(5)：5865-5870.
［16］Lin S，Jiang T，Yu Y，et al.Secernin-1 contributes to colon cancer progression through enhancing matrix met alloproteinase2/9 exocytosis［J］.Dis Markers，2015(2015)：230703.
［17］Zhang G，Miyake M，Lawton A，et al.Matrix met alloproteinase-10 romotes tumor progression through regulation of angiogenic and apoptotic pathways in cervical tumors［J］.BMC Cancer，2014(14)：310.