抗结核骨组织工程复合体局部治疗兔脊柱结核的对比研究

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Abstract Objective To explore the antitubercular bone engineering composite constructed with hydroxyapatite and / β-tricalcium phosphate (HA / β-TCP) composite material and allogenic bone as scaffolds，and to evaluate the effect of two different anti-tubercular bone tissues Complex treatment of spinal tuberculosis in rabbits.Methods Thirty-six New Zealand white rabbits were selected at the age of 3 months and were divided into 3 groups at random.The rabbits in group A were implanted with Rifapentin microspheres-rADSCs /HA / β-TCP anti-TB bone In the tissue engineering complex，group B received Rifapentin microspheres-rADSCs / allogeneic bone antitubercular bone tissue engineering complex at the bone defect.Group C was not treated after debridement.At 4，8 and 12 weeks after operation，the imaging examination (DR) was performed.At the 12th week after operation，the experimental animals were sacrificed and the specimens were taken for general observation.Histopathology was used to observe the repair of bone defects.Results It was found that bone defect in group A was replaced by new bone tissue.The bone defect in group B was basically repaired，and a large amount of bone tissue was formed in the periphery.A small amount of bone tissue was formed in group C，with a large amount of fibrous tissue covered.X-ray observation found：4 weeks，A group of bone defects in a small amount of callus visible，the material in close contact with the surrounding bone tissue.In group B，callus formation was observed in the bone defect area，and the demarcation of the bone defect area in group C was clear and the lamellar low-density shadow was seen.At 8 weeks，the bone defect area of Group A was significantly reduced，the material was absorbed，and the boundary was slightly blurred.B group of bone defect can be seen film-like high-density film，C group of bone defects can be seen point-like calcification.At 12 weeks，the material in group A was basically absorbed，the material in group B was partly absorbed，and the intervertebral space was partly fused.The boundary of group C was still clear and the detail of disc space was destroyed. Histopathological examination found：A group of material absorbed significantly after 12 weeks，showing a large number of fibrous callus tissue to generate bone tissue.B group visible part of allogeneic bone residue，visible a large number of fibrous callus tissue to produce bone tissue.C group shows a large number of fibrous tissue formation.Conclusion The antituberculosis bone engineering complex constructed by Rifapentin microspheres-rADSCs / HA / β-TCP has good biocompatibility and can effectively fill the bone defect after the removal of lumbar tuberculosis in rabbits.

Key words： adipose derived stem cells rifapentin microspheres hydroxyapatite and/β-tricalcium phosphate complexus bone defects

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