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Abstract Objective To observe the relationship of biologicalcharacteristics of amputated limb muscle between with different preservation methods.Methods Quadriceps femoris muscles from Wistar rats werepreserved for 24 hours at 4℃ in extracellular-type trehalose containingKyoto (ETK)，University of Wisconsin (UW)，or lactated Ringer's (LR) solution (control).Amputated limbs of rats preserved with ETK，UW，or LR for6 or 24 hours at 4℃ were transplanted orthotopically.Blood samples from the external jugular vein were obtained 60 minutes after surgery for analysis of serumlevels of creatine phosphokinase (CPK)，aspartate aminotransferase (AST)，alanine aminotransferase(ALT)，lactate dehydrogenase (LDH)，creatinine (Cr)，bloodurea nitrogen (BUN)，and potassium (K).At week 8，terminallatency and amplitude were measured in the tibialis anterior muscle.Themuscles were also analyzed histologically by hematoxylin and eosin staining (HE) and Masson's trichrome staining.Results In the 6-hour-preserved limb transplantation model，electrical amplitude was significantly lower inthe LR group.Histologically，muscles preserved with LR showed the mostatrophic changes.In the 24-hour-preserved model，the survival rate of the LR group was 37.5％ in contrast to 80％ in the ETK and UW groups.Electrical signals were not detected in the LR group owing to severe muscle atrophy and fibrosis.The ETK and UW groups showed good muscle functionelectrophysiologically.Conclusion ETK solutions can protect muscle function and morphology in ischemia-reperfusion limbs and improve recipient survivalrates after transplantation of long-term-preserved limbs.
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［1］Prucz RB，Friedrich JB.Upper extremity replantation：current concepts［J］.Plast Reconstr Surg Glob，2014，133(2)：333-342.
［2］Guerrero A，Gibson K，Kralovich K A，et al.Limb loss following lower extremity arterial trauma：what can be done proactively?［J］.Injury，2002，33(9)：765-769.
［3］McCutcheon C，Hennessy B.Systemic reperfusion injury during arm replantation requiring intraoperative amputation［J］.Anaesth Crit Care，2002，30(1)：71-73.
［4］van der Heijden EPAB，Kroese ABA，Werker PMN，et al.Improving the preservation of isolated rat skeletal muscles stored for 16 hours at 4 degrees C［J］.Transplant，2000，69(7)：1310-1322.
［5］Tsuchida T，Kato T，Yamaga M，et al.The effect of perfusion with UW solution on the skeletal muscle and vascular endothelial exocrine function in rat hindlimbs［J］.J Surg Res，2003，110(1)：266-271.
［6］Tsuji N，Yamashita S，Sugawara Y，et al.Effect of prolonged ischaemic time on muscular atrophy and regenerating nerve fibres in transplantation of the rat hind limb［J］.J Plast Surg Hand Surg，2012，46(34)：217-221.
［7］吕青，周明武，郑喜灿，等.大鼠断肢保存方法与血管组织病理变化的实验研究［J］.解放军护理杂志，2003，20(9)：9-10.
［8］芦笛，刘虎仙，韩岩.威斯康星大学保存液用于大鼠离断肢体低温灌注保存实验［J］.解放军医学院学报，2016 (7)：792-797.
［9］佟晓杰，张彩顺，曹德寿，等.脱细胞异体神经移植物桥接大鼠坐骨神经缺损促进神经-肌结构重建和功能恢复的实验研究［J］.解剖学报，2005，36(1)：1-5.
［10］Lloyd MS，Teo TC，Pickford MA，et al.Preoperative management of the amputated limb［J］.Emerg Med J，2005，22(7)：478-480.
［11］Chin KY，Hart AM.Temporary catheter first perfusion during hand replantation with prolonged warm ischaemia［J］.J Plast Reconstr Aesthet Surg，2012，65(5)：675-677.
［12］Cavadas P C，Ibáñez J，Thione A，et al.Bilateral transhumeral arm transplantation：result at 2 years［J］.Am J Transplant，2011，11(5)：1085-1090.
［13］Juang SE，Huang HW，Kao CW，et al.Effect of university of wisconsin and histidine-tryptophan-ketoglutarate preservation solutions on blood potassium levels of patients undergoing living-donor liver transplantation［J］.Transplantation Proceedings，2012，44(2)：366-368.
［14］Chen F，Nakamura T，Wada H.Development of new organ preservation solutions in Kyoto University［J］.Yonsei Med J，2004，45(6)：1107-1114.
［15］Yoshida H，Okuno H，Kamoto T，et al.Comparison of the effectiveness of ET-Kyoto with Euro-Collins and University of Wisconsin solutions in cold renal storage［J］.Transplant，2002，74(9)：1231-1236.
［16］Canelo R，Hakim NS，Ringe B.Experience with hystidine tryptophan ketoglutarate versus University Wisconsin preservation solutions in transplantation［J］.Int Surg，2003，88(3)：145-151.
［17］Song YX，Muramatsu K，Kurokawa Y，et al.Functional recovery of rat hind-limb allografts［J］.J Reconstr Microsurg，2005，21(7)：471-476. |
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