急性期骨科钛合金板表面金黄色葡萄球菌细菌生物膜的结构演变

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Abstract Objective To observe the biofilm structure of Staphylococcus aureus on the surface of titanium plates at different time points during the acute phase (4 weeks) by laser confocal microscopy and scanning electron microscopy.Methods Staphylococcus aureus was cultured in vitro with orthopaedic titanium plates to form biofilms at different time points.After 1 week，2 weeks，3 weeks and 4 weeks of culture，the titanium plates were taken out，the biofilms points were stained with protein and propidium iodide dyes according to the different time points.After staining，the morphological structure of bacterial biofilms was observed by laser confocal microscope and scanning electron microscope.Results Laser confocal images and electron microscope images of biofilms at different time points were obtained.It was observed that a small amount of extracellular polysaccharide polymer was formed in bacteria at 1 week，and the internal spatial structure was disordered，which indicating the formation of early biofilms.With extension of the culture time，the amount of bacterial exopolysaccharide polymer gradually increased，the internal gaps contact with pores，and the spatial structure became complicated gradually.At 4 weeks，a large number of exopolysaccharide polymers were formed，and the inner membrane structure was wellstructured.It meant that a mature biofilm was formed.The number of viable bacteria in each time point had no difference(P＞0.05)，and the biofilm thickness between each time point had significant difference(P＜0.05).
Conclusion Staphylococcus aureus bacterial biofilm has difference between early and late phase.It is a dynamic evolution processfrom from early biofilm to late mature biofilm，which is manifested in the maturity and stability of bacterial biofilm exopolysaccharide polymers and internal spatial structures.

Key words： staphylococcus aureus biofilm orthopedics internal plants

Corresponding Authors: Niu Peng

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	Chen Mingli
	Chen Zonglin
	Yang Rongyuan
	Xue Guanqi
	Huang Miao
	Niu Peng

Cite this article:

Chen Mingli,Chen Zonglin,Yang Rongyuan, et al. Structural Evolution of Staphylococcus Aureus Biofilm on the Surface of Orthopedic Titanium Plate in Acute Pha[J]. sygkzz, 2023, 29(01): 32-.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2023/V29/I01/32

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