骨髓间充质干细胞旁分泌对成骨细胞生物学活性的影响

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Abstract Objective To investigate the effect of paracrine of bone marrow mesenchymal stem cells (BMSCs) on the biological activity of osteoblasts in bone repair. Methods In order to exclude the influence of BMSCs osteogenic differentiation，we use non-contact co-culture of BMSCs and osteoblastic cells by transwell to observe the changes of BMSCs paracrine on the biological characteristics of MG63 cells.Migration test and scratch test were used to detect the migration and movement ability of osteoblasts.Flow cytometry was used to detect the changes of cell cycle.Alizarin red staining and alkaline phosphatase staining were used to detect the calcification nodule and ALP level of osteoblasts，and RT-qPCR was used to detect the MMP-2，MMP-9，OCN，OPN，Osterix，RUNX2 transcription level.Results In the co-culture environment，the migration and movement ability of osteocytes in the co-culture group were significantly higher than those in the control group (P＜0.05).The proliferation of osteocytes in the co-cultured group was accelerated and the proportion of cells in S phase and G2/M phase in the co-culture group was significantly higher than that in the control group （P＜0.05).The levels of calcified nodules and ALP in co-cultured osteocytes were significantly higher than those in the control group (P＜0.05).The transcriptional level of MMP-2，MMP-9，OPN，Osterix，RUNX2 in co-cultured osteocytes was significantly increased (P＜0.05).Conclusion Under the condition of non-contact co-culture，BMSCs paracrine promotes osteoblast migration，proliferation，division，calcification，ALP activity and multiple osteogenic gene expression.These evidences show that BMSCs not only have the ability of osteogenic differentiation，but also have the paracrine function of enhancing the bone repair activity of surrounding osteoblasts，which provides a new theoretical basis for the application of BMSCs transplantation in bone defect and osteonecrosis.

Key words： bone marrow mesenchymal stem cells osteoblast bone repair paracrine

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