MiR-27b-3p靶向ID-3调控骨肉瘤细胞增殖的研究

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Abstract Objective To observe the targeting relationship between microRNA-27b-3p(miR-27b-3p)and DNA binding/differentiation inhibitory protein-3(ID-3)，and analyze its regulatory effect on proliferation on ostersarcoma cells.Methods Prospective research methods was applicated.Osteosarcoma cell line U20S was selected.Targeting relationship between miR-27b-3p and ID-3 was verified by double luciferase reporter gene assay.The blank control group，empty vector transfection group，miR-27b-3p mimic group，miR-27b-3p mimic and siRNA ID-3 co-transfection group were established.Cell activity was detected by CCK-8 method.Clonal formation was detected by cells colony formation experiment.ID-3 and PCNA were detected by Western Blot method.40 cases of osteosarcoma were selected，including 22 males and 18 females.The average age was(16.3±4.3)years old.Expression of miR-27b-3p was detected by real-time fluorescence quantitative PCR.ID-3 and PCNA were detected by immunohistochemistry method.Results MiR-27b-3p could reduce the luciferase activity in pGL-3-ID-3-WT cells.Compared with the blank control group and the empty vector transfection group，the activity of miR-27b-3p mimic group，the number of colonies and the expression of PCNA decreased.Andthey could be reversed in co transfection group.Negative correlation was found between miR-27b-3p and ID-3，miR-27b-3p and PCNA in osteosarcoma.Positive correlation was found between ID-3 and PCNA in osteosarcoma.The expression of miR-27b-3p was correlated with survival time.Conclusion MiR-27b-3p targeting ID-3 negatively regulates the proliferation on osteosarcoma cells.Lower expression of miR-27b-3p may be related to poor prognosis.

Key words： osteosarcoma microRNA-27b-3p-DNA binding/differentiation inhibitory protein-3 cell proliferation

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