PLGA导管复合骨髓间充质干细胞与细胞外基质凝胶移植修复大鼠坐骨神经缺损的生物力学分析

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Abstract Objective To study the animal model of sciatic nerve injury by combining poly［(lactic-co-glycolic acid)，PLGA］catheters with bone marrow mesenchymal stem cells (bone mesenchymal stem cells，BMSCs) combined with extracellular matrix (extracellular matrix，ECM)，biomechanical properties of the sciatic nerve after gel transplantation and provide basic research support for clinical application.Methods The rat sciatic nerve injury model was transplanted with autologous nerve，PLGA catheter combined with BMSCs，PLGA catheter combined with BMSCs and ECM gel for 6，12，18，and 24 weeks，and the general behavior of rats in each group was observed.The functional index of the sciatic nerve was measured 168 days after the transplantation in the rats，and then the sciatic nerves of the rats in each group were taken for tensile mechanical properties experiments.Results The tensile elastic limit load，elastic limit stress，maximum load，maximum stress，maximum strain，and elastic limit strain of the PLGA catheter combined with BMSCs transplantation group and the PLGA catheter combined with BMSCs and ECM gel transplantation group were greater than those of the autologous nerve transplantation group.The difference was statistically significant (P＜0.05).The tensile elastic limit load，elastic limit stress，maximum load，maximum stress，and elastic limit strain of the PLGA catheter composite BMSCs and ECM gel transplantation group were greater than those of the PLGA catheter composite BMSCs transplantation group.The difference was statistically significant (P＜0.05) Conclusion PLGA catheter combined with BMSCs，PLGA catheter combined with BMSCs and ECM gel transplantation have obvious repair effect on sciatic nerve injury.

Key words： animal model poly(lactic-co-glycolic acid) catheter bone marrow mesenchymal stem cells sciatic nerve damage

Corresponding Authors: Yu Tao

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	Wang Huaicheng
	Li Peng
	Wang Biaowen
	Du Chunyu
	Yu Tao

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Wang Huaicheng,Li Peng,Wang Biaowen, et al. Biomechanical Analysis of Plga Catheter Combined With Bmscs and Extracellular Matrix Gel Transplantation for Repairing Sciatic Nerve Defects in Rats[J]. sygkzz, 2023, 29(2): 132-.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2023/V29/I2/132

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