circPDE4B调控miR-7对骨关节炎中软骨ECM降解和细胞凋亡的影响

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Abstract Objective To investigate the effect and mechanism of circPDE4B regulating miR-7 on the extracellular matrix (ECM) degradation and apoptosis of chondrocyte in osteoarthritis(OA).Methods RT-qPCR was used to detect the expression levels of circPDE4B and miR-7 in OA cartilage tissues and chondrocytes.After OA chondrocytes were transfected with oe-NC and oe-circPDE4B，or si-NC，si-circPDE4B and co-transfected with si-circPDE4B+miR-7 inhibitors，the expression levels of ECM related molecules SOX9，COL2A1，MMP3 and MMP13 were detected by RT-qPCR and Western Blot；and flow cytometry was used to detect cell apoptosis.The dual luciferase reporter gene was used to detect the binding of circPDE4B and miR-7.Results The expression level of circPDE4B in OA cartilage tissues and chondrocytes was significantly lower than that of normal cartilage tissues and chondrocytes (P＜0.05).Overexpression of circPDE4B significantly inhibited the apoptosis of OA chondrocytes and the expression level of apoptosis-related molecule Cleaved-caspase-3 (P＜0.05).Meanwhile，overexpression of circPDE4B significantly promoted the expression levels of SOX9 and COL2A1 (P＜0.05)，and inhibited the expression levels of MMP3 and MMP13 (P＜0.05).The results of the luciferase reporter gene test showed that circPDE4B could target miR-7 (P＜0.05)，and overexpression of circPDE4B inhibited the expression of miR-7 (P＜0.05).The expression levels of circPDE4B and miR-7 in cartilage tissues of patients with OA were negatively correlated (P＜0.05).Co-transfection of si-circPDE4B and miR-7 inhibitors reversed the accelerating effect of single transfection of si-circPDE4B on OA chondrocyte apoptosis and ECM degradation (P＞0.05).Conclusion circPDE4B is significantly low expressed in OA cartilage tissues and chondrocytes.CircPDE4B inhibites OA chondrocyte apoptosis and cartilage ECM degradation by targeting miR-7.

Key words： osteoarthritis circPDE4B miR-7 extracellular matrix apoptosis

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	Articles by authors
	Fan Jinzhu 1
	Ren Xiaoyu 1
	Cong Fei 1
	Song Tao 1
	Guo Yunshan 2
	Ma Qiang 3*

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Fan Jinzhu 1,Ren Xiaoyu 1,Cong Fei 1, et al. circPDE4B Regulates the ECM Degradation and Apoptosis of Chondrocyte Through miR-7 in Osteoarthritis[J]. sygkzz, 2022, 28(6): 512-517.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2022/V28/I6/512

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