高糖环境对软骨细胞RANKL/OPG表达的影响

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Abstract Objective To observe the effect of different glucose concentration on the expression of RANKL and OPG in chondrocytes.Methods Normal chondrocytes came from tibiotalar joint voluntarily donated by patients with car accident and trauma.Chondrocytes were divided into six groups.Normal chondrocytes were cultured in medium containing 5 mmol/L，10 mmol/L，15 mmol/L，20 mmol/L，25 mmol/L and 33 mmol/L glucose.The expression of RANKL and OPG in chondrocytes were detected by Western blot.Results With the increase of glucose concentration，both the expression of RANKL and OPG decreased.The expression of RANKL protein decreased from 2 613.8 to 975.6，and the expression of OPG protein decreased from 3 377.3 to 140.2.However the decrease of OPG expression was much more significant than that of RANKL，and the ratio of RANKL/OPG positively correlated with the increase of glucose concentration.The ratio of RANKL/OPG increased significantly when the glucose concentration was higher than 15 mmol/L.Conclusion With the increase of extracellular glucose concentration，the ratio of RANKL/OPG shows an upward trend.And 15 mmol/L may be the threshold of imbalance of the ratio.This change may be an important factor causing Charcot osteoarthropathy and cartilage damage in diabetic patients.

Key words： high glucose environment chondrocytes receptor activator for nuclear factor-&kappa B ligand osteoprotegerin charcot osteoarthropathy

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	Articles by authors
	Liu Peilong 1
	Zhang Yan 1
	Liang Jingqi 1
	Diao Jiayu 2
	Liu Huan 3
	Liang Xiaojun 1
	Zhang Feng 3
	Zhao Hongmou 1*

Cite this article:

Liu Peilong 1,Zhang Yan 1,Liang Jingqi 1, et al. Effect of High Glucose Environment on the Expression of RANKL/OPG in Chondrocytes[J]. sygkzz, 2022, 27(2): 131-141.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2022/V27/I2/131

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