miR-140-3p靶向凋亡基因BAX减轻白细胞介素-1β诱导的软骨细胞损伤

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Abstract Objective To study the effect and mechanism of miR-140-3p in attenuating interleukin-1 β(IL-1β) induced chondrocyte injury via targeting apoptosis gene Bcl-2 associated X protein (BAX).Methods Rat osteoarticular chondrocytes ATDC5 were cultured and the expression levels of miR-140-3p and BAX were detected after the treatment of different concentration of IL-1β(0，1，5，10ng/mL).Then cells were treated with 10ng/mL IL-1β，at the same time，negative control (NC) miR or miR-140-3p was transfected，NC adenovirus or BAX adenovirus was infected.The cell survival rate，apoptosis rate，miR-140-3p，Bax and cleaved caspase-3 expression levels were detected.The dual luciferase reporter gene experiment was used to verify the effect of miR-140-3p targeting BAX.Results The expression of miR-140-3p decreased and BAX increased in ATDC5 cells treated with different concentrations of IL-1 β(P＜0.05).The survival rate and the expression level of miR-NC+IL-1β group were lower than those of miR-NC group，while the apoptosis rate and the expression levels of BAX and Cleaved caspase-3 were higher than those of miR-NC group(P＜0.05).The survival rate and the expression level of miR-140+IL-1β group were higher than those of miR-NC+IL-1β group，while the apoptosis rate and the expression levels of BAX and Cleaved caspase-3 were lower than those of miR-NC+IL-1β group(P＜0.05).The survival rate of miR-140+Ad-BAX+IL-1β group was lower than that of miR-140+Ad-NC+IL-1β group，while the apoptosis rate and the expression levels of BAX and Cleaved caspase-3 were higher than those of miR-140+Ad-NC+IL-1β group(P＜0.05).Conclusion IL-1β plays a protective role in IL-1β induced chondrocyte injury model，and targeting BAX and inhibiting apoptosis are related molecular mechanisms.

Key words： osteoarthritis interleukin-1β miR-140-3p Bcl-2 related X protein apoptosis

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	Wang Yiwei
	Tian Yongli *
	Ma Guangen
	et al

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Wang Yiwei,Tian Yongli *,Ma Guangen, et al. MiR-140-3p Attenuates Interleukin-1 β Induced Chondrocyte Injury Via Targeting Apoptosis Gene BAX[J]. sygkzz, 2021, 27(12): 1102-1116.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2021/V27/I12/1102

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