CD44分子的体外岩藻糖基化修饰对兔骨髓间充质干细胞归巢能力的影响

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Abstract Objective To investigate the effects of extracorporeal fucosylation of CD44 on the homing ability of rabbit bone marrow mesenchymal stem cells.Methods First，BMSCs were constructed to stably express enhanced green fluorescent protein (EGFP).Then，BMSCs were subjected to fucosylation treatment in vitro.Finally，BMSCs were injected intravenously into the model rabbits with tibial fractures.After 6 weeks of injection，the levels of stromal cell-derived factor (SDF-1) and monocyte chemoattractant protein-1 (MCP-1) in rabbit serum were detected by ELISA.The expression levels of SDF-1 and MCP-1 protein in damaged bone tissue were detected by Western Blot.The positive rate of EGFP expression was detected by staining.Results The results of fluorescence microscopy showed that BMSCs with stable expression of EGFP were successfully constructed.The results of ELISA and Western Blot showed that the secretion of SDF-1 and MCP-1 and the expression of SDF-1 and MCP-1 protein in BMSCs treatment group processed by fucosylated were significantly higher than those in BMSCs treatment group processed byno fucosylated (P＜0.01).The results of immunohistochemical staining showed that the positive rate of EGFP expression was also significantly increased (P＜0.05)，which indicated that the BMSCs at the injured bone tissue were significantly increased and helpful in the repair of bone injury.Conclusion Extracorporeal fucosylationof CD44 molecules can significantly enhance the homing ability of rabbit BMSCs，which may be achieved by SDF-1 and MCP-1 regulation.

Key words： bone mesenchymal stem cells enhanced green fluorescent protein fucosylation bone injury homing ability

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