人自体脂肪间充质干细胞治疗骨关节炎的临床研究

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Abstract Objective To discusses the safety and effectiveness of the treatment injection for knee osteoarthritis by using a utologous ADSCS composite sodium hyaluronate，platelet rich plasma (PRP).Methods Take the abdominal adipose tissue，get hADSCs separation，plenty of ADSCS obtained with in vitro culture，and by flow cytometry instrument to detect the surface markers and analysis into cartilage cells differentiation ability.30 patients with mild-to-moderate of knee joint osteoarthritis were selected from the orthopedic outpatient service center in the first people’s hospital of Xianyang city.ADSCs composite sodium hyaluronate，platelet rich plasma were injected into patient’s articular cavity.The indicators such as patients with adverse reactions，knee activity score，pain visual analogue scale (VAS) score as well as the surface of knee join under MRI were evaluated before and after the intervention.Results Abdominal adipose tissue separated hADSCs were positive expression of CD105 and CD29，CD45，CD34 negative expression，in vitro with good osteogenesis，into fat，cartilage capacity；There was no adverse reaction in the patients who suffered clinical intervention.And patient’s knee pain relief，activity range was improved compared with before treatment.MRI imaging showed that the patchy shadow in the area of the joint surface and low signal area were significantly reduced.There was no obvious joint effusion，and the thickness of articular surface was increased.Conclusion The articular cavity injection therapy with ADSCs compound sodium hyaluronate，platelet rich plasma (PRP) for patients who suffered with osteoarthritis can effectively improved the knee joint motion，to reduce pain and improve the quality of life.The thickness of the joint surface was increased and the joint degeneration was delayed by MRI imaging.

Key words： adipose derived stem cells chondrocytes osteoarthritis

Corresponding Authors: 汪玉良

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