巴氏法联合高渗盐水对骨与软组织恶性肿瘤体外灭活研究

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Abstract Objective To study the effect of 20％ hypertonic saline on the in vitro inactivation of tumor cells and its relationships with temperature and time，and to provide guidance for the inactivation of autologous bone and limb salvage operation in the treatment of clinical malignant tumors of bone and soft tissue.Methods 24 patients who were diagnosed with malignant tumors of bone and soft tissue according to the biopsy of limbs and pelvis before operation were chosen，and 4 patients who didn’t meet the requirement were removed.The samples were treated with 0.9％ saline，20％ hypertonic saline，and pasteurization treatment combined with hypertonic saline (15，30，45 min) and were observed by optical microscope，transmission electron microscope.The inactivation rate of the tumor cells were calculated.Results Inactivated tumor cells at 20％ hypertonic saline and pasteurization combined with hypertonic saline at each time were obviously different compared with 0.9％ isotonic saline，and the difference was statistically significant (P＜0.01).The effect of inactivating of 15 min pasteurization combined with hypertonic saline was better than 20％ hypertonic saline group (P＜0.01).Conclusion The effect of 20％ hypertonic saline on tumor elimination is reliable，and combining with pasteurization or increasing the time of killing tumor cells can obviously increase the necrosis rate of tumor cells.The time of killing tumor cells is more than 30 min，and the effect of killing tumor will be best.

Key words： hypertonic saline malignant bone tumor inactivation apoptosis osteosarcoma

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［1］康晓征，闫万璞，杨永波，等.骨与软组织肉瘤肺转移的单中心大样本外科治疗结果及预后分析［J］.中国肺癌杂志，2016，19(5)：299-306.
［2］郭卫，杨荣利，汤小东，等.复合移植重建恶性骨肿瘤切除后骨缺损［J］.中华骨科杂志，2003，23(4)：13-16.
［3］杨毅，郭卫，杨荣利，等.肿瘤骨灭活再植重建骨盆肿瘤切除后骨缺损的临床研究［J］.中华外科杂志，2014，52(10)：754-759.
［4］彭长亮，杨毅，孙馨，等.高渗盐水灭活自体骨再植的动物实验［J］.北京大学学报(医学版)，2012，44(6)：950-953.
［5］彭秋平，汪森明，张积仁.高渗盐水注射提高对小鼠肝癌冷冻疗效的实验研究［J］.现代诊断与治疗，2002，1(1)：14-16.
［6］Qu H，Guo W，Yang R，et al.Reconstruction of segmental bone defect of long bones after tumor resection by devitalized tumor-bearing bone［J］.World J Surg Oncol，2015，13(1)：282.
［7］赵雨坤，郑康，郭晴晴，等.一种基于EDTA脱钙法的改良骨组织脱钙方法［J］.中国实验方剂学杂志，2016，22(11)：17-19.
［8］刘斌.深低温冷冻灭活技术在骨肉瘤保肢治疗中的应用及进展［J］.中国癌症防治杂志，2016，8(1)：66-68.
［9］张剑英，周滋霞，顾则娟，等.无水乙醇灭活体外肿瘤细胞的时间效应研究［J］.中华护理杂志，2012，47(4)：339-340.
［10］MT Record，Jr W Zhang，CF Anderson.Analysis of effects of salts and uncharged solutes on protein and nucleic acid equilibria and processes：a practical guide to recognizing and interpreting polyelectrolyte effects，hofmeister effects，and osmotic effects of salts［J］.Adv Protein Chem，1998(51)：281-353.
［11］DL Beauchamp，M Khajehpour.Studying salt effects on protein stability using ribonuclease T₁as a model system［J］.Biophys Chem，2012(161)：29-38.
［12］陈秉耀，韦兴，李南，等.微波消融治疗下肢长骨骨肉瘤15例随访报告［J］.中国骨与关节杂志，2014，12(4)：272-276.
［13］许宋锋，刘江，聂鑫，等.酒精灭活瘤段骨在重建恶性骨肿瘤骨缺损中的应用［J］.中国骨与关节杂志，2015，4(5)：354-360.
［14］王俊江，田征.高渗盐水在恶性骨肿瘤治疗中的应用［J］.中华医学杂志，2016，96(11)：910-912.
［15］Sun YX，Cheng W，Han X，et al.In vivo experimental study on the effects of fluid in increasing the efficiency of radiofrequency ablation［J］.Asian Pac J Cancer Prev，2014，15(14)：5799-5804.
［16］Bikowski JB，Dumont AM.Lymphangioma circumscriptum：treatment with hypertonic saline sclerotherapy［J］.J Am Acad Dermatol，2005，53(3)：442-444.
［17］Gennari A，Almairac F，Litrico S，et al.Spinal cord compression due to a primary vertebral hydatid disease：a rare case report in metropolitan france and a literature review［J］.Neurochirurgie，2016，62(4)：226-228.
［18］Togral G，Arikan SM，Ekiz T，et al.Musculoskeletal hydatid cysts resembling tumors：a report of five cases［J］.Orthop Surg，2016，8(2)：246-252.
［19］Takata M，Sugimoto N，Yamamoto N，et al.Activity of bone morphogenetic protein-7 after treatment at varioustemperatures：freezing vs.pasteurization vs.allograft［J］.Cryobiology，2011，63(3)：235-239.