脂联素对骨质疏松大鼠椎体磷酸钙骨水泥强化后骨质量及生物力学的影响及机制

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (1180 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的研究脂联素(adiponectin，APN)对骨质疏松大鼠椎体磷酸钙骨水泥(calcium phosphate cement，CPC)强化后骨质量及生物力学的影响及机制。方法采用双侧卵巢切除术制备骨质疏松模型大鼠，共30只，随机分为对照组、1 mg/kg APN组、2 mg/kg APN组，均行第4腰椎CPC强化，7 d后分别给予生理盐水、1 mg/kg APN、2 mg/kg APN局部注射，连续4周。检测第4腰椎骨密度、骨小梁体积分数(bone volume to total volume，BV/TV)、平均骨小梁厚度(trabecular thickness，Tb.Th)、平均骨小梁数目(trabecular number，Tb.N)、最大符合及刚度，碱性磷酸酶(alkaline phosphatase，ALP)、骨钙素(osteocalcin，OCN)、骨桥蛋白(osteopontin，OPN)、磷酸化p38MAPK(phosphorylated p38MPAK，p-p38 MAPK)、磷酸化JNK(phosphorylated JNK，p-JNK)、磷酸化ERK1/2(phosphorylated ERK1/2，p-ERK1/2)的相对表达水平。结果 1 mg/kg APN组、2 mg/kg APN组CPC强化椎体的骨密度、BV/TV、Tb.N、Tb.Th、最大负荷、刚度，ALP、OCN、OPN的mRNA相对表达量及蛋白相对表达量，p-p38MPAK、p-JNK、p-ERK1/2的蛋白相对表达量均高于对照组。结论 APN增强骨质疏松大鼠椎体CPC强化后的骨质量和生物力学强度，激活p38MPAK、JNK、ERK1/2通路是相关的分子机制。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	张鑫
	马朋朋
	刘肃
	李伟
	张麦粒
	赵立春
	张春玲

关键词 ：骨质疏松, 椎体压缩性骨折, 磷酸钙骨水泥, 脂联素, 信号通路

Abstract：Objective To study the effect and mechanism of adiponectin(APN)on bone quality and biomechanics of vertebral body strengthened with calcium phosphate cement(CPC) in osteoporosis rats.Methods Thirty osteoporosis model rats were prepared by bilateral oophorectomy.They were randomly divided into control group，1 mg/kg APN group and 2 mg/kg APN group.All rats were strengthened with CPC of the fourth lumbar vertebra.7 days later，they were given local injection of normal saline，1 mg/kg APN and 2mg/kg APN respectively for 4 weeks.Bone mineral density，trabecular volume fraction(BV/TV)，average trabecular thickness(Tb.Th)，average trabecular number(Tb.N)，maximum coincidence，stiffness and relative expression levels of alkaline phosphatase(ALP)，osteocalcin(OCN)，osteopontin(OPN)，phosphorylated p38MAPK(pp38 MAPK)，phosphorylated JNK(pJNK)，phosphorylated ERK1/2(pERK1/2)were measured.Results Bone mineral density，BV/TB，Tb.Th，Tb.N，maximum coincidence，stiffness and mRNA relative expression levels of ALP，OCN，OPN and protein relative expression levels of ALP，OCN，OPN，p-p38mpak，p-JNK and p-ERK1/2 were higher than those of the control group.Conclusion APN enhances the bone mass and biomechanical strength of vertebral body strengthened with calcium phosphate cement in osteoporosis rats，and the activation of p38MAPK，JNK，ERK1/2 pathways are related molecular mechanisms.

Key words： osteoporosis vertebral compression fracture calcium phosphate bone cement adiponectin signal pathway

基金资助:河北省卫生健康委员会科研基金项目(20220612)

通讯作者: 张春玲

作者简介: 张鑫(1984－ )，男，主治医师，河北北方学院附属第一医院骨外科，zhangxin1984099@163.com

引用本文:

张鑫，马朋朋，刘肃，李伟，张麦粒，赵立春，张春玲. 脂联素对骨质疏松大鼠椎体磷酸钙骨水泥强化后骨质量及生物力学的影响及机制[J]. 实用骨科杂志, 2022, 28(12): 1084-.

链接本文:

http://www.sygkzz.com/CN/ 或 http://www.sygkzz.com/CN/Y2022/V28/I12/1084

［1］王智强，林路，陈萧霖，等.经皮椎体强化治疗骨质疏松性椎体压缩性骨折：导航定位、骨折复位系统、骨水泥渗漏及材料的改良［J］.中国组织工程研究，2022，26(4)：631-636.
［2］Tian Y，Liu H，He L，et al.Calcium phosphatebased composite cement：Impact of starch type and starch pregelatinization on its physicochemical properties and performance in the vertebral fracture surgical models in vitro［J］.J Biomed Mater Res B Appl Biomater，2021，109(12)：2068-2078.
［3］徐德睿，孟宪荣，路艳艳，等.应用于椎体成形术的骨水泥研究进展［J］.国际老年医学杂志，2020，41(4)：270-273.
［4］王洋，王良宸，万丽娟，等.重组人脂联素对SD大鼠胫骨骨折愈合过程的影响［J］.解放军医学院学报，2021，42(2)：197-201；206.
［5］张磊，唐晓菊，黄有荣，等.聚甲基丙烯酸甲酯骨水泥及磷酸钙骨水泥的材料性能及改性的研究进展［J］.广西医学，2019，41(16)：2114-2118，2122.
［6］de Lacerda Schickert S，Pinto JC，Jansen J，et al.Tough and injectable fiber reinforced calcium phosphate cement as an alternative to polymethylmethacrylate cement for vertebral augmentation：A biomechanical study［J］.Biomater Sci，202，8(15)：4239-4250.
［7］Lu Q，Liu C，Wang D，et al.Biomechanical evaluation of calcium phosphatebased nanocomposite versus polymethylmethacrylate cement for percutaneous kyphoplasty［J］.Spine J，2019，19(11)：1871-1884.
［8］Jeong J，Kim JH，Shim JH，et al.Bioactive calcium phosphate materials and applications in bone regeneration［J］.Biomater Res，2019，14(23)：4.
［9］Parasaram V，Chowdhury A，Karamched SR，et al.Bisphosphosphonate-calcium phosphate cement composite and its properties［J］.Biomed Mater Eng，2019，30(3)：323-331.
［10］Wu X，Tang Z，Wu K，et al.Strontium-calcium phosphate hybrid cement with enhanced osteogenic and angiogenic properties for vascularised bone regeneration［J］.J Mater Chem B，2021，9(30)：5982-5997.
［11］Cui X，Huang C，Chen Z，et al.Hyaluronic acid facilitates bone repair effects of calcium phosphate cement by accelerating osteogenic expression［J］.Bioact Mater，2021，6(11)：3801-3811.
［12］Song B，Fu H，Liu J，et al.Bioactive small molecules in calcium phosphate scaffold enhanced osteogenic differentiation of human induced pluripotent stem cells［J］.Dent Mater J，2021，40(3)：615-624.
［13］Zeng J，Lin J，Yao G，et al.Effect of modified compound calcium phosphate cement on the differentiation and osteogenesis of bone mesenchymal stem cells［J］.J Orthop Surg Res，2017，12(1)：102.
［14］Wang Y，Zhang X，Shao J，et al.Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway［J］.Sci Rep，2017，7(1)：3652.
［15］Liu H，Liu S，Ji H，et al.An adiponectin receptor agonist promote osteogenesis via regulating bonefat balance［J］.Cell Prolif，2021，54(6)：e13035.
［16］Yang S，Liu H，Liu Y，et al.Effect of adiponectin secreted from adipose-derived stem cells on bone-fat balance and bone defect healing［J］.J Tissue Eng Regen Med，2019，13(11)：2055-2066.
［17］Fang H，Judd RL，Fang H，et al.Adiponectin regulation and function［J］.Compr Physiol，2018，8(3)：1031-1063.
［18］Yong J，von Bremen J，RuizHeiland G，et al.Adiponectin interacts in-vitro with cementoblasts influencing cell migration，proliferation and cementogenesis partly through the MAPK signaling pathway［J］.Front Pharmacol，2020，22(11)：585346.
［19］Khawaja K，Frommer KW，Bausch M，et al.Compensation of adiponectin-induced adenosine monophosphate-activated protein kinase and p38 mitogen-activated protein kinase signaling in rheumatoid arthritis synovial fibroblasts［J］.J Interferon Cytokine Res，2021，41(5)：177-186.