Analysis of NGS Detection in 57 Surgical Patients with Spinal Infection
1.Department of Orthopaedics,Shanghai Deji Hospital Affiliated to Qingdao University
2.Department of Laboratory Medicine,Shanghai Deji Hospital Affiliated to Qingdao University
3.Department of Orthopaedics,Changzheng Hospital Affiliated to Naval Medical University
Abstract:Objective To investigate the clinical value of next-generation sequencing technology (NGS) in the treatment of spinal infection.Methods The clinical data of 57 patients with spinal infection who underwent open surgery in Shanghai Deji Hospital Affiliated to Qingdao University from June 2018 to December 2020 were collected for a retrospective comparative analysis,including 41 males and 16 females.The patients aged 21 to 76,with an average age of (55.32±12.56) years old.According to the infection sites,there were 10 cases of cervical spine,20 cases of thoracic spine,and 27 cases of lumbar spine.Routine microbial culture and NGS testing were used for the lesion tissue,and the positive rate of the test report and the time of the test report were observed.Results The pathogens were detected by NGS in the lesions of 38 patients,and the positive rate was 66.7%,including 12 cases of Staphylococcus,6 cases of Streptococcus,5 cases of Enterococcus,2 cases of Klebsiella pneumoniae,2 cases of Escherichia coli,1 case of Brucella,8 cases of fungi,12 cases of virus,and 12 cases of Mycobacterium tuberculosis.The detection cycle was 24 to 36 hours.Among the 10 patients,the pathogenic bacteria were cultured by routine microbial culture,and the positive rate was 26.3%,including 4 cases of Staphylococcus,2 cases of Streptococcus,2 cases of Enterococcus,1 case of Escherichia coli,and 1 case of fungi.The detection period was 3 to 7 days.Compared with conventional microbial culture,the positive rate of NGS detection was significantly increased,and the detection reporting time was significantly reduced,and the difference was statistically significant (P<0.001).Conclusion NGS can detect the pathogens of spinal infection more quickly and accurately,which provides an important basis for early clinical treatment of pathogens.
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