The Comparative Research of Knees’ Articular Surface Cartilage and Loose Body’s Surface Cartilage Using Fourier Transform Infrared Spectroscopic Imaging
1.Department of Orthopedics，Central Hospital of Xuzhou
2.China-Japan Fellowship Hospital
Abstract：Objective To study the difference between articular surface cartilage and loose body’s surface cartilage.Methods The knees’ articular surface cartilage and loose body’s surface cartilage were collected research objects，using Fourier Transform Infrared Spectroscopic Imaging and Principle Component Analysis to explore the different of the two samples.Results There was no different in the total PGs content of the two samples calculated by an indirection method.N-H bending vibration wavenumber of collagen in the second derivative peaks (in articular surface cartilage is 1 514 cm－1，in the loose body is 1 516 cm－1) and N-H bending vibration and C-N stretching vibration wavenumber of Amide Ⅱ in the second derivative peaks (in articular cartilage is 1 573 cm－1，in the loose body is 1 575 cm－1) showing up an obvious blue shift.PCA shows that the articular surface cartilage was mainly distributed in the first quadrant，and the loose body was mainly distributed in the three or four quadrant.Conclusion The component of loose body surface cartilage，especially collagen has some deficient compared with articular surface cartilage.
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