Objective  To discuss the anatomical characteristics of “H” point of adult knee joints，so as to enrich the anatomical content around the knee joint and provide theoretical basis for 3D printing osteotomy guide plate.Methods  Five adult knee specimens were provided by the department of anatomy，of Hebei medical university.The average age was (64.6±5.5).There were 4 males and 1 female.The anteromedial part of the knee joint was dissected layer by layer，and anatomic marks were made with positioning needle.The shape of “H” point and the course of surrounding soft tissue were observed and recorded.The relevant anatomical parameters of “H” point were measured and recorded for qualitative and quantitative description.Results  The “H” point was found in all the 5 knee specimens，which was located in the posteromedial tibia，close to the medial tibial ridge，and was about 1.53 cm from the articular surface. The vertical distance between the “H” point and the standard HTO incision was 3.53 cm.The “H” point was an irregular ellipse with an average transverse diameter of (0.62±0.07)cm.Conclusion  The “H” point is the insertion point of semimembranosus muscle tendon，which is about 1.53 cm below the tibial plateau.The existence of “H” point provides a stable and effective anatomical basis for 3D printing HTO osteotomy guide plate，and plays a certain role in digital knee preservation treatment.