高糖环境对软骨细胞RANKL/OPG表达的影响

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摘要目的通过细胞实验观察不同糖浓度对软骨细胞中核因子κB受体活化因子配体(receptor activator for nuclear factor-κ B ligand，RANKL)与骨保护素(osteoprotegerin，OPG)表达的影响。方法 8例正常软骨，男5例，女3例；年龄29~55岁，平均(41.3±12.6)岁。均来自于因车祸及严重外伤需要截肢患者的胫距关节。将软骨细胞分为6组，使用糖浓度为5 mmol/L、10 mmol/L、15 mmol/L、20 mmol/L、25 mmol/L、33 mmol/L的培养基培养正常软骨细胞，通过Western Blot方法检测软骨细胞RANKL与OPG的表达量。结果随着糖浓度的升高，RANKL与OPG表达量均有所减少，RANKL蛋白与OPG蛋白的表达量均呈逐渐减小趋势，RANKL蛋白表达量由2 613.8降低至975.6，OPG蛋白表达量由3 377.3降低至140.2，但是OPG的表达量相较于RANKL减少更显著；RANKL/OPG比值与糖浓度呈正相关，糖浓度>15 mmol/L时RANKL/OPG比值显著上升。结论随着细胞外糖浓度的增加，RANKL/OPG的比值呈上升趋势，15 mmol/L可能为比例失衡的阈值，这种变化可能是造成糖尿病患者出现夏科氏关节病及软骨破坏的重要因素。

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	刘培珑 1
	张言 1
	梁景棋 1
	刁佳宇 2
	刘欢 3
	梁晓军 1
	张峰 3
	赵宏谋 1*

关键词 ：高糖环境, 软骨细胞, 核因子κB受体活化因子配体, 骨保护素, 夏科氏骨关节病

Abstract：Objective To observe the effect of different glucose concentration on the expression of RANKL and OPG in chondrocytes.Methods Normal chondrocytes came from tibiotalar joint voluntarily donated by patients with car accident and trauma.Chondrocytes were divided into six groups.Normal chondrocytes were cultured in medium containing 5 mmol/L，10 mmol/L，15 mmol/L，20 mmol/L，25 mmol/L and 33 mmol/L glucose.The expression of RANKL and OPG in chondrocytes were detected by Western blot.Results With the increase of glucose concentration，both the expression of RANKL and OPG decreased.The expression of RANKL protein decreased from 2 613.8 to 975.6，and the expression of OPG protein decreased from 3 377.3 to 140.2.However the decrease of OPG expression was much more significant than that of RANKL，and the ratio of RANKL/OPG positively correlated with the increase of glucose concentration.The ratio of RANKL/OPG increased significantly when the glucose concentration was higher than 15 mmol/L.Conclusion With the increase of extracellular glucose concentration，the ratio of RANKL/OPG shows an upward trend.And 15 mmol/L may be the threshold of imbalance of the ratio.This change may be an important factor causing Charcot osteoarthropathy and cartilage damage in diabetic patients.

Key words： high glucose environment chondrocytes receptor activator for nuclear factor-&kappa B ligand osteoprotegerin charcot osteoarthropathy

基金资助:西安市卫健委面上培育项目(2021ms07)
西安市科技计划项目(2019115013YX005SF038(12))

通讯作者: 赵宏谋

作者简介: 刘培珑(1991－ )，男，医师，西安交通大学附属红会医院足踝外科，1219604798@qq.com.

引用本文:

刘培珑 1，张言 1，梁景棋 1，刁佳宇 2，刘欢 3，梁晓军 1，张峰 3，赵宏谋 1*. 高糖环境对软骨细胞RANKL/OPG表达的影响[J]. 实用骨科杂志, 2022, 27(2): 131-141.
Liu Peilong 1，Zhang Yan 1，Liang Jingqi 1，Diao Jiayu 2，Liu Huan 3，Liang Xiaojun 1，Zhang Feng 3，Zhao Hongmou 1*. Effect of High Glucose Environment on the Expression of RANKL/OPG in Chondrocytes. sygkzz, 2022, 27(2): 131-141.

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http://www.sygkzz.com/CN/ 或 http://www.sygkzz.com/CN/Y2022/V27/I2/131

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