远隔缺血后适应对老龄大鼠骨折愈合的影响及研究

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Abstract Objective To clarify whether remote ischemic post-conditioning can promote the expression of various factors in the process of fracture healing，and thus promote fracture healing.Methods 64 aged rats(18~20 months old)were randomly divided into two groups.The ischemic postconditioning group was treated with limb remote ischemic post-conditioning and the control group was blank.At 7，14，28，42 days after fracture，rats were sacrificed subsequent.Micro-computed tomography was performed to measure healing of the bone tissue and biomechanical testing was used to test mechanical strength.In addition，the two primary angiogenic mediators，HIF-1α and vascular endothelial growth factor，as well as the osteoblast markers runt-related transcription factor 2，alkaline phosphatase and osteocalcin，were determined at the mRNA and protein levels by reverse transcription-quantitative polymerase chain reaction and western blot analysis.Results The callus volume and gray value of the hypoxia treatment group were significantly higher than those in the control group at 14，28 days and 42 days after operation(P＜0.01).The maximal force，stiffness and work to fracture of the tibial specimens in the hypoxic treatment group were significantly higher than those in the control group(P＜0.05).The expression of HIF-1α protein was significantly higher than that of the control group(P＜0.01).The protein expression levels of ALP，OCN and Runx2 in the remote ischemic post-conditioning group were significantly higher than those in the control group at three time points.Conclusion Fracture after short-term appropriate frequency of ischemic post-ischemic adaptation can promote aged rat tibial fracture healing，its role is to promote osteogenesis and promote angiogenesis in two ways to achieve.

Key words： fracture healing remote ischemic post-conditioning hypoxia induction factor

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