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Abstract Objective To assess the osteogenic differentiation in the condition of co-culture of BMSC and ADSC.Methods ADSC and BMSC obtained under sterile conditions.Oil red O was used to identify two cells after being induced into fat.Toluidine blue staining was used to identify two cells after being induced into Chondrocytes.Alizarin red staining were used in osteogenic induction group.Osteogenic induction group was divided into four sub-groups，group A：BMSC＋ADSC uninduced group(BMSC：ADSC＝1︰1)；group B：BMSC induced group；group C：ADSC induced group；group D：BMSC＋ADSC induced group(BMSC：ADSC＝1︰1).OCN and RUNX2 mRNA expression were detected by qPCR after osteogenic induction for 14 days，while the protein expression of OCN and RUNX2 were tested by Western blot at the same time.Results Oil red O staining，Toluidine blue staining and Alizarin red staining showed positive results.The mRNA expression and protein expression of OCN and RUNX2 were significiantly higher than the other three groups after osteogenic induction for 14 days in vitro.Conclusion The ability of osteogenic potential strengthened to some extent under the condition of co-culturing.
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