miR-140在不同阶段骨关节炎软骨细胞中的表达规律及功能

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Abstract Objective To investigate the expression miR-140 in different stage of osteoarthritis and its function after transfecting ds-miR-140 mimic/inhibitor into chondrocytes. Methods Cartilage was classified into three groups，normal group，mid-staged group and advanced-staged group，according to the Kellgren and Lawrence criterion.6-8 cases of normal or OA cartilage were collected and chondrocytes were isolated.The second generation of chondrocytes were used to test.The levels of miR-140，COL₂A1 and COL10A1 mRNA were measured with real-time fluorescent quantitative PCR.The ds-miR-140 mimic/inhibitorwere transfected into the mid-staged group，and the expression of COL₂A1，COL10A1 and SOX9 were measured.Results Compared with the normal group，miR-140 expression in the mid- and advanced-staged group was decreased by 34.1％ and 49.3％ respectively，and its expression in advanced-staged group was decreased by 73.9％.Beside，SOX9 expression in the mid- and advanced-staged group was decreased by 75％ and 82.7％respectively，COL₂A1 expression decreased by 36.7％ and 78.7％ respectively，while COL10A1 expression increased to 1.66 times and 2.10 times respectively.After transfecting ds-miR-140 mimic/inhibitor into the mid-staged group，SOX9 expression was increased by 1.95 times or decreased by 59.1％，COL₂A1 expression was increased by 1.87 times or decreased by 68.8％，and COL10A1 expression was decreased by 73.0％ or increased by 1.96 times.Conclusion The expression level of miR-140 decreased in osteoarthritic chondrocytes and was negatively related to the severity of OA.Overexpression its level by transfecting ds-miR-140 mimic into chondrocytes could up-regulate SOX9 and COL-2A1 expression while down-regulate COL10A1 expression.

Key words： osteoarthritis chondrocyte microRNA-140 collagen type Ⅱ collagen type X

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