Beclin 1和MAP1-LC3在家兔激素性股骨头缺血坏死中表达的实验研究

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Abstract Objective To investigate the expression of autophagy-related gene Beclin 1 and microtubule-associated protein 1 light chain 3(MAP1-LC3)in avascular necrosis of the femoral head model induced by glucocorticoids，and explore the role of autophagy in avascular necrosis of the femoral head model induced by glucocorticoids. Methods Forty mature Japanese rabbits were randomly divided into experimental group(n＝28)and control group(n＝12).The experimental group was given an intramscular injection of 7.5 mg/kg of methylprednisolone twice per week for 8 weeks in all；the control group was given equal isotonic Na chloride.Rabbits of each group were killed at the 8th week respectively，and the hibateral heads of femur were taken out.The left heads of femur were made into general sections，dyed by HE.The expression of Beclin-1 and MAP1-LC3 were detected by immunohistochemical method.The right heads of femur were put into liquid nitrogen quickly，protein were extracted from bone tissue，right head of femur was detected the protein expression of Beclin 1 and MAP1-LC3 by Western blot.Results Hematoxylin and eosin stain showed the successfuluccessful production of steroid induced avascular necrosis of the femoral head in rabbits.The expression of Beclin l and MAPI-LC3 in the experimental group were 96.43％and 92.86％ respectively，and the expression of Beclinl and MAPI-LC3 in the control group were 16.67％and 8.33％ respectively.And the difference was significant (P＜0.05).Compared to the control group，the protein expression of Beclinl and MAPI-LC3 increased remarkably in the experimental group，the difference was significant (P＜0.05).Conclusion Adrenal cortex hormones cause the rabbits early avascular necrosis of the femoral head，Beclin 1 and MAP1-LC3 expression increased.This study suggests that autophagy may play an important role in the development of femoral head model induced by glucocorticoids.

Key words： gucocorticoids osteonecrosis autophagy Beclin 1 microtubule-associated protein 1 light chain 3

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	Wang Wenxuan 1
	Zhao Zhenqun 2
	Liu Wanlin 2
	et al

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Wang Wenxuan 1,Zhao Zhenqun 2,Liu Wanlin 2, et al. An Experimental Study on the Expression of Beclin 1 and MAP1-LC3 in Avascular Necrosis of the Femoral Head Induced by Glucocorticoids[J]. sygkzz, 2017, 23(9): 811-815.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2017/V23/I9/811

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