导航模板注射锂基生物玻璃水凝胶修复股骨头坏死的效应研究

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (20164 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract

Objective (1)To explore lithium-based bioglass hydrogels and engender mesoporous bioglass biomaterials with multiple biomedical functions such as bone promotion，angiogenesis promotion and lipogenesis inhibition.(2)3D printing technology was used to make navigation templates，accurately locate and remove the lesions of osteonecrosis of the femoral head(ONFH)，and lithium-based biogalglass hydrogel was accurately injected to observe its effect on repairing ONFH.Methods GelMA and lithium bioglass hydrogel (GM/M-Li) were prepared by using Gelatin (Gelatin) and methacrylic anhydride (MA).The materials were characterized by scanning electron microscope (SEM) and transmission electron microscope(TEM).At the same time，the biocompatibility of the material was evaluated by cell alive/dead staining，cell counting kit-8 (CCK-8) and cell morphology observation.In addition，immunofluorescence (IF) staining，alkaline phosphatase (ALP) staining，alizarin red staining (ARS)，angiogenesis assay，and oil red O staining were used to evaluate the osteogenic differentiation ability，angiogenesis ability，and lipogenesis inhibition ability of the materials.A rabbit femoral head necrosis model was successfully established in vivo.

The necrotic bone was removed by 3D navigation template combined with core decompression and then filled with GM/M-Li hydrogel.Hematoxylin eosin(HE) staining，oil red O staining and immunohistochemical staining were used to verify the osteogenesis，angiogenesis and lipogenesis inhibition.Results The results of SEM showed that the GM/M-Li hydrogel has a three-dimensional porous structure.The results of TEM showed that M-Li material was in nanometer scale.The ion release experiments show that the lithium element in GM/M-Li hydrogel has slow release effect.It was proved that GM/M-Li hydrogel had good biocompatibility by cell alive/dead staining and CCK-8.In vitro experiments on osteogenesis，angiogenesis and inhibition of adipogenesis confirmed that the release of lithium ions in the material was conducive to the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCS) and the tubule formation of human umbilical vein vascular endothelial cells (HUVECs)，and inhibited the adipogenesis of adipogenic precursor cells (3T3-L1).In vivo HE staining and IF proved that GM/M-Li hydrogel effectively promoted bone tissue and blood vessel regeneration.The ability of GM/M-Li hydrogel to inhibit fat was verified by oil red O staining.Conclusion GM/M-Li can be accurately injected into the necrotic area of the femoral head and cleared the lesion with 3D printed navigation template.GM/M-Li hydrogel has good biocompatibility and can promote the reconstruction of bone defect in osteonecrosis of the femoral head by regulating BMSCS differentiation in osteogenic direction，promote the formation of blood vessels and reduce the infiltration of fat cells.

Key words： navigation template osteonecrosis of the femoral head hydrogel bioglass osteogenesis

Corresponding Authors: Zhang Chuankai

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Ma Qiaoqiao
	Wu Zerui
	Zha Guochun
	Guo Kaijin
	Jiang Shouhai
	Zhang Chuankai

Cite this article:

Ma Qiaoqiao,Wu Zerui,Zha Guochun, et al. Study on the Effect of Injection of Lithium Bioglass Hydrogel with Navigation Template to Repair Femoral Head Necrosis[J]. sygkzz, 2024, 30(1): 39-.

URL:

http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2024/V30/I1/39

［1］ Chang C，Greenspan A，Gershwin ME.The pathogenesis，diagnosis and clinical manifestations of steroid-induced osteonecrosis［J］.J Autoimmun，2020(110)：102460.
［2］ Luo P，Gao F，Han J，et al.The role of autophagy in steroid necrosis of the femoral head：A comprehensive research review［J］.Int Orthop，2018，42(7)：1747-1753.
［3］ Fukushima W，Fujioka M，Kubo T，et al.Nationwide epidemiologic survey of idiopathic osteonecrosis of the femoral head［J］.Clin Orthop Relat Res，2010，468(10)：2715-2724.
［4］ Wang A，Ren M，Wang J.The pathogenesis of steroid-induced osteonecrosis of the femoral head：A systematic review of the literature［J］.Gene，2018(671)：103-109.
［5］ Li D，Xie X，Yang Z，et al.Enhanced bone defect repairing effects in glucocorticoid-induced osteonecrosis of the femoral head using a porous nano-lithium-hydroxyapatite/gelatin microsphere/erythropoietin composite scaffold［J］.Biomater Sci，2018，6(3)：519-537.
［6］ Wang H，Fu X，Shi J，et al.Nutrient element decorated polyetheretherketone implants steer mitochondrial dynamics for boosted diabetic osseointegration［J］.Adv Sci (Weinh)，2021，8(20)：e2101778.
［7］ Lee RSB，Hamlet SM，Moon HJ，et al.Re-establishment of macrophage homeostasis by titanium surface modification in type II diabetes promotes osseous healing［J］.Biomaterials，2021(267)：120464.
［8］ Schumacher M，Habibovic P，Van Rijt S.Mesoporous bioactive glass composition effects on degradation and bioactivity［J］.Bioact Mater，2021，6(7)：1921-1931.
［9］ Zheng K，Boccaccini AR.Sol-gel processing of bioactive glass nanoparticles：A review［J］.Adv Colloid Interface Sci，2017(249)：363-373.
［10］ Huang L，Yin X，Chen J，et al.Lithium chloride promotes osteogenesis and suppresses apoptosis during orthodontic tooth movement in osteoporotic model via regulating autophagy［J］.Bioact Mater，2021，6(10)：3074-3084.
［11］ Peng F，Qiu L，Yao M，et al.A lithium-doped surface inspires immunomodulatory functions for enhanced osteointegration through PI3K/AKT signaling axis regulation［J］.Biomater Sci，2021，9(24)：8202-8220.
［12］ Geng D，Wu J，Shao H，et al.Pharmaceutical inhibition of glycogen synthetase kinase 3 beta suppresses wear debris-induced osteolysis［J］.Biomaterials，2015(69)：12-21.
［13］ Li N，Bai J，Wang W，et al.Facile and versatile surface functional polyetheretherketone with enhanced bacteriostasis and osseointegrative capability for implant application［J］.ACS Appl Mater Interfaces，2021，13(50)：59731-59746.
［14］李坤，刘佳，李芳芳，等.3D打印导航模板辅助微创髓芯减压联合可注射人工骨治疗早期股骨头坏死的临床研究［J］.中国医学创新，2021，18(22)：9-12.
［15］ Guo G，Zhang H，Shen H，et al.Space-selective chemodynamic therapy of CuFe5O8 nanocubes for implant-related infections［J］.ACS Nano，2020，14(10)：13391-13405.
［16］ Liu Y，Chan-Park MB.A biomimetic hydrogel based on methacrylated dextran-graft-lysine and gelatin for 3D smooth muscle cell culture［J］.Biomaterials，2010，31(6)：1158-1170.
［17］ Zhao X，Sun X，Yildirimer L，et al.Cell infiltrative hydrogel fibrous scaffolds for accelerated wound healing［J］.Acta Biomater，2017(49)：66-77.
［18］ Nichol JW，Koshy ST，Bae H，et al.Cell-laden microengineered gelatin methacrylate hydrogels［J］.Biomaterials，2010，31(21)：5536-5544.
［19］ Aubin H，Nichol JW，Hutson CB，et al.Directed 3D cell alignment and elongation in microengineered hydrogels［J］.Biomaterials，2010，31(27)：6941-6951.
［20］ Xin T，Mao J，Liu L，et al.Programmed sustained release of recombinant human bone morphogenetic protein-2 and inorganic ion composite hydrogel as artificial periosteum［J］.ACS Appl Mater Interfaces，2020，12(6)：6840-6851.
［21］ Lin R，Deng C，Li X，et al.Copper-incorporated bioactive glass-ceramics inducing anti-inflammatory phenotype and regeneration of cartilage/bone interface［J］.Theranostics，2019，9(21)：6300-6313.
［22］ Yang N，Sun H，Xue Y，et al.Inhibition of MAGL activates the Keap1/Nrf2 pathway to attenuate glucocorticoid-induced osteonecrosis of the femoral head［J］.Clin Transl Med，2021，11(6)：e447.
［23］ Yamamoto T，Irisa T，Sugioka Y，et al.Effects of pulse methylprednisolone on bone and marrow tissues：Corticosteroid-induced osteonecrosis in rabbits［J］.Arthritis Rheum，1997，40(11)：2055-2064.
［24］ El-Rashidy AA，Roether JA，Harhaus L，et al.Regenerating bone with bioactive glass scaffolds：A review of in vivo studies in bone defect models［J］.Acta Biomater，2017(62)：1-28.
［25］ Yim RL，Lee JT，Bow CH，et al.A systematic review of the safety and efficacy of mesenchymal stem cells for disc degeneration：Insights and future directions for regenerative therapeutics［J］.Stem Cells Dev，2014，23(21)：2553-2567.
［26］ Wu X，Sun W，Tan M.Noncoding RNAs in Steroid-Induced Osteonecrosis of the Femoral Head［J］.Biomed Res Int，2019(2019)：8140595.
［27］ Yang Q，Yin W，Chen Y，et al.Betaine alleviates alcohol-induced osteonecrosis of the femoral head via mTOR signaling pathway regulation［J］.Biomed Pharmacother，2019(120)：109486.
［28］ Yu Z，Fan L，Li J，et al.Lithium prevents rat steroid-related osteonecrosis of the femoral head by beta-catenin activation［J］.Endocrine，2016，52(2)：380-390.
［29］ Xia K，Yu LY，Huang XQ，et al.Epigenetic regulation by long noncoding RNAs in osteo-adipogenic differentiation of mesenchymal stromal cells and degenerative bone diseases［J］.World J Stem Cells，2022，14(1)：92-103.
［30］ Bai J，Li L，Kou N，et al.Low level laser therapy promotes bone regeneration by coupling angiogenesis and osteogenesis［J］.Stem Cell Res Ther，2021，12(1)：432.
［31］ Pluharova E，Baer MD，Mundy CJ，et al.Aqueous cation-amide binding：free energies and ir spectral signatures by ab initio molecular dynamics［J］.J Phys Chem Lett，2014，5(13)：2235-2240.
［32］ Loi F，Cordova LA，Pajarinen J，et al.Inflammation，fracture and bone repair［J］.Bone，2016(86)：119-130.
［33］ Guo R，Yamashita M，Zhang Q，et al.Ubiquitin ligase Smurf1 mediates tumor necrosis factor-induced systemic bone loss by promoting proteasomal degradation of bone morphogenetic signaling proteins［J］.J Biol Chem，2008，283(34)：23084-23092.
［34］ Sabareeswaran A，Basu B，Shenoy SJ，et al.Early osseointegration of a strontium containing glass ceramic in a rabbit model［J］.Biomaterials，2013，34(37)：9278-9286.
［35］ Poh PS，Hutmacher DW，Stevens MM，et al.Fabrication and in vitro characterization of bioactive glass composite scaffolds for bone regeneration［J］.Biofabrication，2013，5(4)：045005.
［36］ Naruphontjirakul P，Porter AE，Jones JR.In vitro osteogenesis by intracellular uptake of strontium containing bioactive glass nanoparticles［J］.Acta Biomater，2018(66)：67-80.
［37］ Tang H，Lu Z，Xue G，et al.The development and understanding of intracranial aneurysm based on rabbit model［J］.Neuroradiology，2020，62(10)：1219-1230.