膝关节表面软骨与游离体表面软骨FT-IR光谱成像的对比研究

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1168 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To study the difference between articular surface cartilage and loose body’s surface cartilage.Methods The knees’ articular surface cartilage and loose body’s surface cartilage were collected research objects，using Fourier Transform Infrared Spectroscopic Imaging and Principle Component Analysis to explore the different of the two samples.Results There was no different in the total PGs content of the two samples calculated by an indirection method.N-H bending vibration wavenumber of collagen in the second derivative peaks (in articular surface cartilage is 1 514 cm^－1，in the loose body is 1 516 cm^－1) and N-H bending vibration and C-N stretching vibration wavenumber of Amide Ⅱ in the second derivative peaks (in articular cartilage is 1 573 cm^－1，in the loose body is 1 575 cm^－1) showing up an obvious blue shift.PCA shows that the articular surface cartilage was mainly distributed in the first quadrant，and the loose body was mainly distributed in the three or four quadrant.Conclusion The component of loose body surface cartilage，especially collagen has some deficient compared with articular surface cartilage.

Key words： articular cartilage fourier transform infrared spectroscopic loose body principle component analysis

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2017/V23/I4/328

［1］Li Y，Cai H，Fang W，et al.Fibroblast growth factor 2 involved in the pathogenesis of synovial chondromatosis of temporomandibular joint［J］.J Oral Pathol Med，2014，43(5)：388-394.
［2］Wright JK，Cawston TE，Hazleman BL.Transforming growth factor beta stimulates the production of the tissue inhibitor of metalloproteinases (TIMP) by human synovial and skin fibroblasts［J］.Biochim Biophys Acta，1991，1094(2)：207-210.
［3］Wright JK，Cawston TE，Hazleman BL.Transforming growth factor beta stimulates the production of the tissue inhibitor of metalloproteinases (TIMP) by human synovial and skin fibroblasts［J］.Biochim Biophys Acta，1991，1094(2)：207-210.
［4］裴明，曲绵域，于长隆，等.骨关节炎关节软骨及游离体中胶原Ⅰ、Ⅱ、Ⅲ型的表达［J］.中华风湿病学杂志，2000，4(2)：85-88.
［5］Arokoski JP，Jurvelin JS，Väätäinen U，et al.Normal and pathological adaptations of articular cartilage to joint loading［J］.Scand J Med Sci Sports，2000，10(4)：186-198.
［6］Rieppo L，Rieppo J，Jurvelin JS，et al Fourier transform infrared spectroscopic imaging and multivariate regression for prediction of proteoglycan content of articular cartilage［J］.PloS One，2012，7(2)：1-8.
［7］Mosher TJ，Liu Y，Torok CM.Functional cartilage MRI T2 mapping：evaluating the effect of age and training on knee cartilage response to running［J］.Osteoarthritis Cartilage，2010，18(3)：358-364.
［8］Boskey A，Pleshko CN.FT-IR imaging of native and tissue-engineered bone and cartilage［J］.Biomaterials，2007，28(15)：2465-2478.
［9］Camacho NP，West P，Torzilli PA，et al.FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage［J］.Biopolymers，2001，62(1)：1-8.
［10］Kim M，Bi X，Horton WE，et al.Fourier transform infrared imaging spectroscopic analysis of tissue engineered cartilage：histologic and biochemical correlations［J］.J Biomed Opt，2005，10(3)：031105.
［11］Jackson M，Choo LP，Watson PH，et al.Beware of connective tissue proteins：assignment and implications of collagen absorptions in infrared spectra of human tissues［J］.Biochim Biophys Acta，1995，1270(1)：1-6.
［12］Servaty R，Schiller J，Binder H，et al.Hydration of polymeric components of cartilage-an infrared spectroscopic study on hyaluronic acid and chondroitin sulfate［J］.Int J Biol Macromol，2001，8(2)：121-127.
［13］Kim M，Foo LF，Uggen C，et al.Evaluation of early osteochondral defect repair in a rabbit model utilizing fourier transform-infrared imaging spectroscopy，magnetic resonance imaging，and quantitative T2 mapping［J］.Tissue Eng Part C Methods，2010，16(3)：355-364.
［14］Kohler A，Bertrand D，Martens H，et al.Multivariate image analysis of a set of FTIR microspectroscopy images of aged bovine muscle tissue combining image and design information［J］.Anal Bioanal Chem，2007，389(4)：1143-1153.
［15］Kim M，Foo LF，Uggen C，et al.Evaluation of early osteochondral defect repair in a rabbit model utilizing fourier transform-infrared imaging spectroscopy，magnetic resonance imaging，and quantitative T2 mapping［J］.Tissue Eng Part C Methods，2010，16(3)：355-364.
［16］Hanifi A，McCarthy H，Roberts S，et al.Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues［J］.PLoS ONE，2013，8(5)：e64822.
［17］Yorgancigi H，Karahan N，Baydar ML.Multiple loose bodies in the joints：from snowstorm to hailstones［J］.J Arthroscopy，2004，20(8)：113-116.
［18］Pascual-Garrido C，Tanoira I，Muscolo DL，et al.Viability of loose body fragments in osteochondritis dissecans of the knee.A series of cases［J］.Int Orthop，2010，34(6)，827-831.
［19］Saotome K，Tamai K，Osada D，et al.Histologic classification of loose bodies in osteoarthrosis［J］.J Orthop Sci，2006，11(6)：607-613.
［20］Touten Y，Adachi N，Deie M，et al.Histologic evaluation of osteochondral loose bodies and repaired tissues after fixation［J］.Arthroscopy，2007，23(2)：188-196.
［21］Giannini S，Buda R，Grigol B，et al.The detached osteochondral fragment as a source of cells for autologous chondrocyte implantation (ACI) in the ankle joint［J］.Osteoarthritis Cartilage，2005，13(7)：601-607.
［22］Swingler TE，Wheeler G，Carmont V，et al.The expression and function of micro RNAs in chondrogenesis and osteoarthritis［J］.Arthritis Rheum，2012，(64)6：1909-1919.
［23］Nagase H，Visse R，Murphy G.Structure and function of matrix metalloproteinases and TIMPs［J］.Cardiovasc Res，2006，69(3)：562-573.
［24］Visse R，Nagase H.Matrix metalloproteinases and tissue inhibitors of metalloproteinases：structure，function，and biochemistry［J］.Cir Res，2003，92(8)：827-839.

[1]	. [J]. sygkzz, 2021, 27(5): 441-443.