miR-133a靶向MMP-14抑制骨肉瘤细胞侵袭的作用及机制研究

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Abstract Objective To study the effect and mechanism of miR-133a on inhibiting osteosarcoma cell invasion via targeting matrix metalloproteinase-14 (MMP-14). Methods Normal osteoblasts hFOB1.19 and osteosarcoma cells MG63，U2OS and SAOS2 were cultured and the expression of miR-133a was detected by fluorescence quantitative PCR.MG63 cells were divided into miR-negative control (NC) group，miR-133a group，pcDNA3.1+miR-NC group，pcDNA3.1+miR-133a group，pcDNA3.1-MMP-14+miR-133a group and transfected with NC mimic，miR-133a mimic，pcDNA3.1 plasmid and MMP-14 overexpressed pcDNA3.1 plasmid.Thenthe number of cell invasion was detected by Transwell，the expression level of MMP-14 was detected by Western blot and the targeted binding of miR-133a to 3'UTR of MMP-14 gene mRNA was verified by double luciferase reporter gene assay.Results The expression level of miR133a in MG63，U2OS and SAOS2 cells were lower than that in hFOB1.19 cell，and the expression level of miR-133a in MG63 cells decreased most significantly.The invasion number of MG63 cells in miR-133a group was less than that in miR-NC group.The expression level of MMP-14 and the fluorescence activity of double luciferase reporter gene containing wild type 3'UTR of MMP-14 were lower than those in miR-NC group.The invasion number of MG63 cells in pcDNA3.1-MMP-14+miR-133a group was more than that in pcDNA3.1+miR-133a group.Conclusion miR-133a can inhibit the invasion of osteosarcoma MG63 cells，and the targeted inhibition of MMP-14 geneis one of the possible molecular mechanism.

Key words： osteosarcoma miR-133a matrix metalloproteinase-14 invasion

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	Articles by authors
	Huang Shengxiang
	Mei Haibo*
	Liu Kun
	Tang Jin
	Wu Jiangyan

Cite this article:

Huang Shengxiang,Mei Haibo*,Liu Kun, et al. Effect and Mechanism of miR-133a Inhibiting Osteosarcoma Cell Invasion By Targeting MMP-14[J]. sygkzz, 2022, 28(1): 36-40.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2022/V28/I1/36

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