N-乙酰半胱氨酸对软骨细胞基质降解和骨关节炎的作用研究

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Abstract Objective To investigate the effect of N-acetylcysteine(NAC) on chondrocyte matrix degradation and osteoarthritis.Methods The model of chondrocytes induced by interleukin(IL)-1β was established in vitro，and the cell viability was measured by tetramethylazozolium salt(methyl thiazolyl tetrazolium，MTT) method and stained with pyridine iodide.Superoxide detection(SOD) kit，total glutathione peroxidase(GPX) detection kit wereused to detect intracellular ROS，SOD and GPX content.The expression of matrix metalloproteinase(MMP)-3，-9，-13 and ADAMTS 4 were detected by Western blot.Chondrocyte matrix was detected by Alcin blue staining.A rat model of osteoarthritis was established by right knee anterior cruciate ligament resection in vivo，and the mechanical pain threshold and knee joint swelling diameter of rats were detected.RT-PCR was used to detect the expression levels of MMP-4，MMP-9 and MMP-13 in rat synovial tissues.Safranin O staining was used to detect the pathological changes in rat synovial tissues.Results NAC can significantly inhibit the production of ROS，SOD and GPX induced by IL-1β in chondrocytes，as well as down-regulating the expression of MMP-4，MMP-9，MMP-13 and ADAMTS 4.In addition，NACcouldalso inhibit chondrocyte matrix degradation in vitro.NAC significantly increased the threshold of mechanical pain and reduced joint swelling in OA rats.RT-PCR results showed that NAC significantly down-regulated the espression of MMP-4，MMP-9，and MMP-13 in synovial tissue of OA rats.Safranin O staining showed that NAC significantly relieved the destruction and degradation of joint matrix in OA rats.Conclusion N-acetylcysteine can inhibit IL-1βinduced oxidative stress-mediated chondrocyte matrix degradation，and it has potential therapeutic value for osteoarthritis.

Key words： N-acetylcysteine osteoarthritis metal matrix protease

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	Zhang Weihua
	Zhang Jianye
	Ye Heng
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Zhang Weihua,Zhang Jianye,Ye Heng, et al. Effect of N-acetylcysteine on Chondrocyte Matrix Degradation and its Effect on Osteoarthritis[J]. sygkzz, 2021, 27(2): 137-142.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2021/V27/I2/137

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